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Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary

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Abstract

A cell line, designated NOCC, was established from the ascites of a patient with clear cell adenocarcinoma of the ovary. The cell line has been grown without interruption and continuously propagated by serial passaging (more than 76 times) over 7 years. The cells are spherical to polygonal-shaped, display neoplastic, and pleomorphic features, and grow in a jigsaw puzzle-like pattern while forming monolayers without contact inhibition. The cells proliferate rapidly, but are easily floated as a cell sheet. The population doubling time is about 29 h. The number of chromosomes ranges from 60 to 83. The modal number of chromosomes is 70–74 at the 30th passage. NOCC cells secreted 750.5 ng/ml of VEGF over 3 days of culture. Hypoxia inducible factor-1α (HIF-1α) is a primary regulator of VEGF under hypoxic conditions. NOCC cells were not sensitive to the anticancer drugs BEV, DOX, GEM, ETP, CDDP, or TXT. The graft of NOCC cells to a scid mouse displayed similar histological aspects to the original tumor. Both the NOCC cells and the graft of the NOCC cells gave a positive PAS reaction.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (B) [No. 20390501 (H.I.)], and a Grant-in-Aid for challenging Exploratory Researches [No. 26670908 and No. 22659314 (H.I.)].

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Authors and Affiliations

  1. Department of NDU Life Sciences, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan

    Akihiro Ohyama, Junko Toyomura, Haruka Takahashi & Hiroshi Ishikawa

  2. Division of Fine Morphology, Core Research Facilities, The Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato-ku, Tokyo, 105-0082, Japan

    Toshiaki Tachibana

  3. Department of Obstetrics and Gynecology, Jikei Daisan Hospital, 4-11-1 Izumihoncho, Komae-shi, Tokyo, 201-8601, Japan

    Seiji Isonishi

  4. Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-1500, Japan

    Haruka Takahashi

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  1. Akihiro Ohyama
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  2. Junko Toyomura
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  3. Toshiaki Tachibana
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  4. Seiji Isonishi
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  5. Haruka Takahashi
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  6. Hiroshi Ishikawa
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Correspondence to Akihiro Ohyama.

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Ohyama, A., Toyomura, J., Tachibana, T. et al. Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary. Human Cell 29, 188–196 (2016). https://doi.org/10.1007/s13577-016-0142-x

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  • DOI: https://doi.org/10.1007/s13577-016-0142-x

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