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1BL/1RS translocation in durum wheat and its effect on end use quality traits

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Abstract

The gluten proteins document the genotypic identity of a wheat variety, in addition to providing valuable clues about its ancestry and technological properties. In this study, an Indian durum wheat genotype B662 was identified to carry 1BL/1RS translocation and characterized further for its effect on end use quality traits. Comparison of the end use quality traits of B662 with five other durum cultivars without 1BL/1RS, showed decreased gluten content, lower swelling index of glutenins and low MSDS-SV indicating that, B662 with 1BL/1RS is not good for pasta making. In F2:3 seeds from a durum wheat cross between the 1BL/1RS cultivar B662 and HI8498 without the translocation, the secalin Sec-1 loci segregated in theoretically expected 3:1 proportion and were inherited as a block of the rye chromosome arm. The analysis of F2:3 harvests for the two most important durum wheat quality tests showed that the presence of 1BL/1RS translocation did not alter the grain protein content values, but was associated with significant reduction of micro SDS-sedimentation volume indicating inferior quality, thus limiting the commercial exploitation of durum wheat genotypes with 1BL/1RS translocation. The cautious use of rye translocation in Indian durum wheat breeding is suggested.

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Abbreviations

PC:

Protein content

HMW-GS:

High molecular weight glutenin subunits

LMW-GS:

Low molecular weight glutenin subunits

SDS:

Sodium dodecyl sulphate

MSDS-SV:

Micro sodium dodecyl sulphate sedimentation volume

PAGE:

Polyacrylamide gel electrophoresis

NIT:

Near Infrared Transmittance

SSDSSV:

Specific SDSSV

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Acknowledgments

Authors wish to thank Dr. HN Pandey, Ex Head, IARI Regional wheat Research Station, Indore, 452 001 for providing seeds of the genotypes used in the study.

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  1. Genetics and Plant Breeding Group, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411 004, India

    Manoj D. Oak & Shubhada A. Tamhankar

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  1. Manoj D. Oak
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  2. Shubhada A. Tamhankar
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Correspondence to Manoj D. Oak.

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Oak, M.D., Tamhankar, S.A. 1BL/1RS translocation in durum wheat and its effect on end use quality traits. J. Plant Biochem. Biotechnol. 26, 91–96 (2017). https://doi.org/10.1007/s13562-016-0366-6

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  • DOI: https://doi.org/10.1007/s13562-016-0366-6

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