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Isolation and analysis of the promoter of IbMYB1 gene from storage roots of purple-fleshed sweet potato

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Abstract

In purple-fleshed sweet potato, transcription factors were isolated such as IbMADS10, IbMYB and WD40. IbMYB1 activates the expression of the structural anthocyanin genes and controls anthocyanin biosynthesis specifically in the flesh of storage roots. However, the mechanisms controlling IbMYB1 in underground organs remain unresolved. A 1186-bp promoter sequence of IbMYB1 by hiTAIL-PCR was isolated, the cis-regulatory elements of the promoter sequence were analyzed by using the PLACE and PlantCARE databases. Many cis-regulatory elements associated with sucrose, gibberellins and light were found in the promoter region, such as, SURE1STPAT21, WBOXHVISO1, PYRIMIDINEBOXHVEPB1 and WRKY71OS. The results from GUS histochemical staining indicated that the promoter drove the expression of the reporter gene and induced constitutive GUS expression in all tissues throughout the growth period. The IbMYB1 promoter responses to sucrose, methyl jasmonate and gibberellins treatments. It was obviously up-regulated by 100 μM methyl jasmonate treatment. These results suggest that the ability of plant hormones to modulate the anthocyanin biosynthetic pathway is highly dependent on the IbMYB1 promoter.

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Abbreviations

MYB:

v-myb avian myeloblastosis viral oncogene homolog

SR:

storage roots

bHLH:

basic helix-loop-helix

MJ:

Methyl jasmonate

GAs:

Gibberellins

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Acknowledgments

The authors are grateful to the support by the China National Natural Science Foundation (grant number 31171601) and Guangdong Natural Science Foundation (grant number 10151063101000038). The authors thank Dr. Defeng Wu for his technical assistance.

Author contributions statement

Wei Dong and Feng Gao designed the research. Wei Dong and Liangliang Niu performed experiments. Wei Dong, Haihang Li and Feng Gao analysed the results. Wei Dong, Haihang Li and Feng Gao drafted the manuscript. Feng Gao conceived and coordinated the study.

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Funding

This study was funded by the China National Natural Science Foundation (grant number 31171601) and Guangdong Natural Science Foundation (grant number 10151063101000038).

Conflict of interest

The authors declare that we have no conflict of interest.

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Authors and Affiliations

  1. Guangdong Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, People’s Republic of China

    Wei Dong, Liangliang Niu, Haihang Li & Feng Gao

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  1. Wei Dong
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  2. Liangliang Niu
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  3. Haihang Li
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  4. Feng Gao
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Correspondence to Feng Gao.

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Dong, W., Niu, L., Li, H. et al. Isolation and analysis of the promoter of IbMYB1 gene from storage roots of purple-fleshed sweet potato. J. Plant Biochem. Biotechnol. 25, 278–284 (2016). https://doi.org/10.1007/s13562-015-0339-1

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  • DOI: https://doi.org/10.1007/s13562-015-0339-1

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