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Gene expression changes in tomato during symptom development in response to leaf curl virus infection

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Abstract

During stress conditions, plant gene expression changes to cope up with the altered environment. The reprogramming of gene expression during viral infections is to be accounted towards host adaptation to the invading virus by developing defense mechanism. As a consequence of restructured gene expression, plant’s physiology gets affected and is evident in the form of symptoms. In the present study, Tomato leaf curl New Delhi virus (ToLCNDV) has been used as model system for the study of altered expression of genes in tomato during symptom development using microarray. Gene expression profiles were monitored in leaf tissues during symptom development in Solanum lycopersicum. To explore the differential expression analysis, tomato cDNA gene chip arrays have been used with 26,173 protein-coding genes. A total of 920 differentially expressed genes in response to ToLCNDV were identified in tomato. KEGG pathway analysis of these genes categorizes 652 differentially expressed genes into 77 known pathways, mostly related to increasing respiration rate, decreasing rate of photosynthesis, accumulation of soluble sugars/starch and results in elevated levels of amino acid synthesis. The possible role of these changes in altering host physiology, developed in the form of symptoms has been discussed.

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Abbreviations

ROS:

Reactive oxygen species

AOX:

Alternative oxidase

JA:

Jasmonic acid

SA:

Salicylic acid

ET:

Ethylene

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Acknowledgments

This work was supported by ICAR funding under NPTC project on transgenic tomato. We are also thankful to Department of Biotechnology, Government of India for providing fellowship to Harpreet Kaur and C.B. Yadav.

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Authors and Affiliations

  1. Advanced Center for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Harpreet Kaur, Chandra Bhan Yadav, P. Jyothsna, V. G. Malathi & Shelly Praveen

  2. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Abdulrahman A. Alatar & Mohammad Faisal

  3. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Shelly Praveen

Authors
  1. Harpreet Kaur
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  2. Chandra Bhan Yadav
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  3. Abdulrahman A. Alatar
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  4. Mohammad Faisal
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  5. P. Jyothsna
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  6. V. G. Malathi
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  7. Shelly Praveen
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Corresponding author

Correspondence to Shelly Praveen.

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Harpreet Kaur and Chandra Bhan Yadav contributed equally

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Kaur, H., Yadav, C.B., Alatar, A.A. et al. Gene expression changes in tomato during symptom development in response to leaf curl virus infection. J. Plant Biochem. Biotechnol. 24, 347–354 (2015). https://doi.org/10.1007/s13562-014-0280-8

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  • DOI: https://doi.org/10.1007/s13562-014-0280-8

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