Abstract
Superoxide dismutases (SODs), members of the metalloenzymes family are most effective intracellular enzymatic antioxidant in aerobic organisms. These enzymes provide the first line of defense in plants against the toxic effects of elevated levels of reactive oxygen species (ROS) generated during various environmental stresses. The availability of high-throughput computational tools has provided better opportunities to characterize the protein features and determine their function. In the present study an attempt was made to gain an insight into the structure and evolution of subunits of SODs (Cu-Zn, Mn and Fe SODs) of rice. The 3-Dimensional structures of SODs were modeled based on available X-ray crystal structures and further validated. The primary sequence, secondary and tertiary structure analysis revealed Mn and Fe SOD to be structurally homologous while Cu-Zn SOD is un-related to either of them. Comparative structural study also revealed former two were dominated by α-helices followed by β-strands in contrast; Cu-Zn SOD dominated by β-strands. Molecular phylogeny indicated a common evolutionary origin of Mn and Fe SOD while Cu-Zn SOD may have evolved separately.
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Abbreviations
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
- RMSD:
-
Root mean square deviation
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Acknowledgement
The authors thankfully acknowledge the financial support from Department of Information Technology, Ministry of Communications and Information Technology, Government of India, New Delhi as well Biotechnology Information System Network, Department of Biotechnology, Government of India.
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Dehury, B., Sarma, K., Sarmah, R. et al. In silico analyses of superoxide dismutases (SODs) of rice (Oryza sativa L.). J. Plant Biochem. Biotechnol. 22, 150–156 (2013). https://doi.org/10.1007/s13562-012-0121-6
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DOI: https://doi.org/10.1007/s13562-012-0121-6