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Proteomic analysis of Daphnia magna exposed to caffeine, ibuprofen, aspirin and tetracycline

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Abstract

The toxicity of pharmaceuticals thrown indiscriminately adversely affects the environment. In this study, the impact of caffeine, ibuprofen, aspirin and tetracycline has been examined through toxicity assay based proteomic analysis. Proteomic profile in Daphnia magna was analyzed by two-dimensional electrophoresis with pH in the range 3 to 10. The LC50 of caffeine, ibuprofen, aspirin and tetracycline determined as 445.3, 91.5, 310 and 90 mg/L, respectively, was used to expose to D. magna grown for 48 hour before isolating total proteins. The results show that the expression pattern of protein’s profile in D. magna gets changed due to the toxicity of caffeine, ibuprofen, aspirin and tetracycline. A large number of proteins expressed differently in D. magna in comparison to those exposed to various pharmaceuticals. The results showed that 18 up- and 1 down-regulation spot in relation to caffeine exposure; 24 up- and 6 down-regulation spots in relation to ibuprofen exposure; 3 up- and 4 down-regulation spots in relation to aspirin exposure and 10 up- and 3 down-regulation spots in relation to tetracycline exposure have been observed. Thus, the present systemic approach using proteomic analysis is very helpful in screening novel biomarkers for pharmaceuticals induced hazardous effects.

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Authors and Affiliations

  1. Department of Bioprocess Engineering, Chonbuk National University, 664-14 Deokjin-dong 1ga Deokjin-gu, Jeonju, 561-756, Korea

    Seung Hyuck Bang, Nam-Hui Hong & Jiho Min

  2. Department of Microbiology, Chungbuk National University, Chungdae-ro 1, Seowon-gu, Cheongju, 362-763, Korea

    Ji-Young Ahn, Simranjeet Singh Sekhon & Yang-Hoon Kim

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  1. Seung Hyuck Bang
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  2. Nam-Hui Hong
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  3. Ji-Young Ahn
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  4. Simranjeet Singh Sekhon
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  5. Yang-Hoon Kim
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  6. Jiho Min
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Correspondence to Yang-Hoon Kim or Jiho Min.

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These authors contributed equally to this work.

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Bang, S.H., Hong, NH., Ahn, JY. et al. Proteomic analysis of Daphnia magna exposed to caffeine, ibuprofen, aspirin and tetracycline. Toxicol. Environ. Health Sci. 7, 97–104 (2015). https://doi.org/10.1007/s13530-015-0226-0

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  • DOI: https://doi.org/10.1007/s13530-015-0226-0

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