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Effect of Micromonospora sp. KSC08 on nitrogen conservation throughout composting

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Abstract

Composting includes a series of reactions resulting in alterations on organic content and nitrogen amount. NH3 volatilization via ammonification and N2 and N2O losses through nitrification are the major sources of nitrogen loss during composting. Amendment with microorganism inoculation was among recently adopted approaches to compensate for nitrogen losses and improve compost quality. Compost amendment via Micromonospora sp. KSC08, an asymbiotic free-living-(N2)-fixing microorganism, was conducted in the present study in order to investigate microorganism’s potential for nitrogen regulation. Twenty windrow systems with varying olive pomace%, microorganism amount, and addition time were prepared for statistical determination of the changes in total C, total nitrogen, and nitrate-nitrogen amounts. Analyses were evaluated in accordance with pH, humidity, and temperature measurements obtained during composting. Final part of the study included maturity evaluation and PCA modeling of FT-IR data. Micromonospora sp. KSC08 was shown to improve microbial activity and regulate nitrogen content by providing exogenous nitrogen to compost mixture. PCA models revealed entirely different structures between untreated and Micromonospora sp. KSC08-treated compost samples at the end of 120 days. The variant molecular structure of samples inoculated with Micromonospora sp. KSC08 was attributed to reactions between carboxyclic acid units and nitrogenous compounds leading to a significant increase in amide content compared to untreated mixtures. Higher amide content was due to higher nitrogen content of Micromonospora sp. KSC08-treated compost, and based on the findings, it was concluded that Micromonospora sp. KSC08 had been effective in nitrogen regulation and proposed as a possible component of microbial consortium for use in conventional composting systems.

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Code availability

Statistical analyses were conducted via Minitab software. OPUS 5.5 (Bruker, USA) software was utilized in the analysis of spectral data. Principal component analysis (PCA) was done by Camo Software, NO.

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Funding

This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK 217O238) and Bilecik Seyh Edebali University Research Fund (2018–02. BŞEÜ.25–02).

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Authors and Affiliations

  1. Biotechnology Application and Research Center, Bilecik Seyh Edebali University, Bilecik, Turkey

    Ayten Kumas, Saadet Gizem Ertekin, Rafig Gurbanov, Yunus Emre Simsek, Fadime Ozdemir Kocak & Levent Degirmenci

  2. Department of Molecular Biology and Genetics, Bilecik Seyh Edebali University, Bilecik, Turkey

    Rafig Gurbanov

  3. Department of Chemical Engineering, Bilecik Seyh Edebali University, Bilecik, Turkey

    Yunus Emre Simsek & Levent Degirmenci

  4. Department of Nursing, School of Health, Bilecik Seyh Edebali University, Bilecik, Turkey

    Fadime Ozdemir Kocak

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  1. Ayten Kumas
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Correspondence to Levent Degirmenci.

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Kumas, A., Ertekin, S.G., Gurbanov, R. et al. Effect of Micromonospora sp. KSC08 on nitrogen conservation throughout composting. Biomass Conv. Bioref. 13, 2375–2390 (2023). https://doi.org/10.1007/s13399-021-01662-z

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