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Cell Aggregating Temperament and Biopotency of Cultivable Indigenous Actinobacterial Community Profile in Chicken (Gallus gallus domesticus) Gut System

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Abstract

The aim of the present study is to explore the population density and diversity of cultivable gut actinobacterial flora from chicken faeces and assess their probiotic attributes. The cultivation-dependent method revealed that occurrence of total actinobacterial population density ranged between 3.20 and 6.55 log CFU \(\hbox {gfw}^{-1}\). Totally, 108 actinobacteria were isolated from faeces of indigenous chickens, among which 50 (GD1–GD50) were chosen according to their distinctive morphological phenotypes and growth rate. The diversity profile of cultivable actinobacterial community divulges that Streptomyces had a higher frequency of distribution (30%) followed by Nocardiopsis (14%), Pseudonocardia (12%), Kitasatospora (10%), Thermoactinomyces (8%), Actinomadura (8%), Kibdelosporangium (6%), Thermomonospora (4%), Saccharopolyspora (4%), whereas Actinokineospora and Actinopolyspora showed low percentage of frequency (2%). The actinobacterial cultures (\({n}=50\)) were screened for colonization ability and beneficial activity (production of functional dietary enzymes and antibacterial activity) which are essential for the screening of indigenous probionts to ensure better colonization and exert beneficial effects on the host. The results depict that among the 50 actinobacterial isolates, 23 (40%) showed high aggregation profile and only 15 (30%) isolates exhibited constructive results to produce multiple dietary exoenzymes. The nine isolates revealing excellent multiple inhibitory activities against all the tested bacterial pathogens were subjected to multicriteria decision analysis wherein GD5 and GD18 ranked as the prominent probionts. Together, the present findings suggest that the chicken faeces may represent an unexplored reservoir of novel cultivable actinobacteria with a potential target for probiotics and bioprospecting for novel bioactive molecules.

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Acknowledgements

GV is grateful to DST (Department of Science and Technology), New Delhi, Government of India, for the award of INSPIRE fellowship (IF 140963/DST/INSPIRE Fellowship/2014/Dt.30.12.2014). SL is indebted to DST-SERB (Department of Science and Technology-Science and Engineering Research Board), New Delhi, Government of India, for the National Post-Doctoral fellowship (PDF/2016/003456/Dt.28.03.2017). All the authors thank the DST-FIST (Department of Science and Technology-Fund for Improvement of S&T Infrastructure) programme for providing instrument facility (DST Sanction Order No-SR/FIST/LSI-013/2012/Dt.13.08.2012). DD is thankful to the UGC (University Grants commission), New Delhi, Government of India, for the financial support in the form of Raman Fellowship for Post-Doctoral Research in the USA (F.no: 5-29/2016(IC), Dt.10.02. 2016). Special mention to Dr. P.M. Gopinath for his valuable suggestions in performing MCDA analysis.

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Authors and Affiliations

  1. Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    Gopal Vinothini, Rajendran Kavitha & Dharumadurai Dhanasekaran

  2. Department of Petrochemical Technology, University College of Engineering, Bharathidasan Institute of Technology (BIT) Campus, Anna University, Tiruchirappalli, Tamil Nadu, 620024, India

    Selvanathan Latha & Muthukumarasamy Arulmozhi

  3. Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH, 03824, USA

    Dharumadurai Dhanasekaran

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  1. Gopal Vinothini
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Vinothini, G., Kavitha, R., Latha, S. et al. Cell Aggregating Temperament and Biopotency of Cultivable Indigenous Actinobacterial Community Profile in Chicken (Gallus gallus domesticus) Gut System. Arab J Sci Eng 43, 3429–3442 (2018). https://doi.org/10.1007/s13369-018-3083-8

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