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High levels of divergent HIV-1 quasispecies in patients with neurological opportunistic infections in China

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Abstract

Despite the fact that the survival of people infected with human immunodeficiency virus (HIV) has improved worldwide because of the increasingly powerful and highly active antiretroviral therapy, opportunistic infections (OIs) of the central nervous system (CNS) remain a serious burden. HIV-1 is capable of entering the CNS through infected peripheral monocytes, but its effect on OIs of CNS remains unclear. In this study, we investigated the characteristics of HIV-1 in acquired immunodeficiency syndrome (AIDS) patients with CNS OIs. A total of 24 patients with CNS OIs and 16 non-CNS OIs (control) cases were selected. These AIDS patients were infected with HIV-1 by paid blood donors in China. HIV-1 loads in plasma and cerebrospinal fluid (CSF) were detected using RT-PCR, and the C2-V5 region of HIV-1 envelope gene was amplified from viral quasispecies isolated from CSF using nested PCR. The CSF HIV-1 load of CNS OIs was higher than that of non-CNS OIs, but plasma HIV-1 load of CNS OIs was not higher than that of non-CNS OIs. The nucleotide sequence of C2-V5 region of the HIV-1 quasispecies isolated from the CSF of CNS OIs had a high diversity, and the HIV-1 quasispecies isolated from the CSF of CNS OIs revealed R5 tropism as 11/25 charge rule. These results suggest that high levels of divergent HIV-1 quasispecies in the CNS probably contribute to opportunistic infections.

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Acknowledgments

This work was supported by the Twelfth Key Science and Technology Five Year Plan of China (2012ZX10001-002, 2012ZX10001-003, and 2012ZX10001-004), National Natural Science Foundation of China (30910103915, 81272266, and 30870853), and Beijing Natural Science Foundation (7092045 and 7101005). We also appreciate the Chronic Disease Follow-up System of Beijing You'An Hospital (Beijing AIDS clinical data and sample database, D09050703560903).

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Authors and Affiliations

  1. Department of Infectious Diseases, Beijing You’An Hospital, Capital Medical University, Beijing, 100069, China

    Yulin Zhang, Feili Wei, Lifeng Liu, Sufang Yang, Ronghua Jin & Ning Li

  2. Department of Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan Province, 637000, China

    Qi Liang

  3. Beijing Institute of Hepatology, Capital Medical University, Beijing, 100069, China

    Wei Ding, Luxin Qiao, Fengli Song & Dexi Chen

  4. Department of Medicine, Jiangsu Geriatrics Institute, Jiangsu Geriatric Hospital, Nanjing, 210023, Jiangsu Province, China

    Jianhua Gu

  5. STD AIDS Research Center, Beijing Key Laboratory (No. BZ0089), Beijing You’An Hospital, Beijing Institute of Hepatology, Capital Medical University, Beijing, 100069, China

    Dexi Chen

Authors
  1. Yulin Zhang
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  2. Feili Wei
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  3. Qi Liang
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  4. Wei Ding
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  5. Luxin Qiao
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  6. Fengli Song
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  7. Lifeng Liu
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  8. Sufang Yang
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  9. Ronghua Jin
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  10. Jianhua Gu
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  11. Ning Li
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  12. Dexi Chen
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Corresponding authors

Correspondence to Jianhua Gu, Ning Li or Dexi Chen.

Additional information

Yulin Zhang, Feili Wei, and Qi Liang contributed equally to this work.

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Zhang, Y., Wei, F., Liang, Q. et al. High levels of divergent HIV-1 quasispecies in patients with neurological opportunistic infections in China. J. Neurovirol. 19, 359–366 (2013). https://doi.org/10.1007/s13365-013-0176-8

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  • DOI: https://doi.org/10.1007/s13365-013-0176-8

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