Abstract
Simian varicella virus (SVV) open reading frame (ORF) 63, duplicated in the virus genome as ORF 70, is homologous to varicella zoster virus ORF 63/70. Transfection of bacterial artificial chromosome clones containing the wild-type SVV genome and mutants with stop codons in ORF 70, in both ORFs 63 and 70 and the repaired virus DNA sequences into Vero cells produced a cytopathic effect (CPE). The onset of CPE was much slower with the double-mutant transfectants (10 days vs. 3 days) and plaques were smaller. While SVV ORF 63 is not required for replication in culture, its expression leads to robust virus replication.
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Acknowledgements
This manuscript is dedicated to the memory of Dr. Nambi Parthasarathy, a close friend and a mentor of R.M. This work was supported in part by Public Health Service grants AG032958 (D.G., R.J.C., S.P., R.M.) and AG006127 (D.G.) from the National Institutes of Health. We thank Marina Hoffman for editorial assistance and Cathy Allen for manuscript preparation.
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The authors report no conflicts of interest. The authors alone are responsible for the content and writing the paper.
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Brazeau, E., Wellish, M., Kaufer, B.B. et al. Simian varicella virus open reading frame 63/70 expression is required for efficient virus replication in culture. J. Neurovirol. 17, 274–280 (2011). https://doi.org/10.1007/s13365-011-0025-6
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DOI: https://doi.org/10.1007/s13365-011-0025-6