Skip to main content
SpringerLink
Log in

Development of microsatellite markers for the plant bug, Pachypeltis micranthus (Hemiptera: Miridae)

  • Technical Note
  • Published:
Applied Entomology and Zoology Aims and scope Submit manuscript

Abstract

The plant bug, Pachypeltis micranthus Mu et Liu (Hemiptera: Miridae), is an effective biological agent of the notoriously worldwide invasive weed, Mikania micrantha Kunth (Asterales: Asteraceae). Previous studies concentrated on ecological and biological aspects of this species. In order to facilitate ecological, evolutionary and conservation genetic analyses, we here identified 1787 microsatellites or simple sequence repeats (SSRs) markers in previously generated expressed sequence tags, using next generation sequencing technology. Seventeen SSR markers were used to analyze the polymorphism of 30 P. micranthus individuals. The number of alleles ranged from 2 to 11. The observed (H O) and expected (H E) heterozygosities varied from 0.071 to 0.655 and 0.188 to 0.835, respectively. The polymorphic information content values were from 0.175 to 0.801. Seven loci had significant deviations from Hardy–Weinberg equilibrium after Bonferroni’s correction. These loci will facilitate study on population genetics of this species in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Anderson JA, Churchill GA, Autrique JE, Tanksley SD, Sorrells ME (1993) Optimizing parental selection for genetic linkage maps. Genome 36:181–186

    Article  CAS  PubMed  Google Scholar 

  • Chapuis MP, Estoup A (2007) Microsatellite null alleles and estimation of population differentiation. Mol Biol Evol 24:621–631

    Article  CAS  PubMed  Google Scholar 

  • Hamarsheh O, Amro A (2011) Characterization of simple sequence repeats (SSRs) from Phlebotomus papatasi (Diptera: Psychodidae) expressed sequence tags (ESTs). Parasit Vectors 4:189

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jarne P, Lagoda PJL (1996) Microsatellites, from molecules to populations and back. Trends Ecol Evol 11:424–429

    Article  CAS  PubMed  Google Scholar 

  • Ji M, Ze SZ, Zhao N, Yang B (2014) Effects of Pachypeltis sp. feeding on defense enzyme activities in Mikania micrantha leaves. Acta Agric Zhejiangensis 26:748–751

    Google Scholar 

  • Métais I, Hamon B, Jalouzot R, Peltier D (2002) Structure and level of genetic diversity in various bean types evidenced with microsatellite markers isolated from a genomic enriched library. Theor Appl Genet 104:1346–1352

    Article  PubMed  Google Scholar 

  • Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. Ph.D. thesis, University of Dublin for “The Excel Microsatellite Tool Kit”

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, NY

    Google Scholar 

  • Tomimura K, Inoue H, Iwanami T (2014) Development of simple sequence repeat markers for the Japanese population of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae). Appl Entomol Zool 49:353–357

    Article  Google Scholar 

  • Wang S, Wang X, He Q, Liu X, Xu W, Li L, Gao J, Wang F (2012) Transcriptome analysis of the roots at early and late seedling stages using Illumina paired-end sequencing and development of EST-SSR markers in radish. Plant Cell Rep 31:1437–1447

    Article  CAS  PubMed  Google Scholar 

  • Wang HL, Yang J, Boykin LM, Zhao QY, Wang YJ, Liu SS, Wang XW (2014) Developing conversed microsatellite markers and their implications in evolutionary analysis of the Bemisia tabaci complex. Sci Rep 4:6351

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xu Y, Zhou W, Zhou Y, Wu J, Zhou X (2012) Transcriptome and comparative gene expression analysis of Sogatella furcifera (Horváth) in response to southern rice black-streaked dwarf virus. PLoS ONE 7:e36238

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yang XM, Sun JT, Xue XF, Zhu WC, Hong XY (2012) Development and characterization of 18 novel EST-SSRs from the western flower thrips, Frankliniella occidentalis (Pergande). Int J Mol Sci 13:2863–2876

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ze SZ, Ji M, Yan ZL, Zhou PY, Nong Z (2011) Analysis and identification of hindgut volatiles for Pachypeltis sp. and field lure. Zool Res 32:147–153

    Google Scholar 

  • Ze SZ, Su EG, Yan ZL, Zhan YS, Ji M (2013) Effects of Pachypeltis sp. against Mikania micrantha. J West China Forest Sci 42:46–52

    Google Scholar 

  • Zhao X, Tan Z, Feng H, Yang R, Li M, Jiang J, Shen G, Yu R (2011) Microsatellites in different Potyvirus genomes: survey and analysis. Gene 488:52–56

    Article  CAS  PubMed  Google Scholar 

  • Zhu JY, Li YH, Yang S, Li QW (2013) De novo assembly and characterization of the global transcriptome for Rhyacionia leptotubula using Illumina paired-end sequencing. PLoS ONE 8:e81096

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

We are grateful to Dr. Guo-Qing Liu working at the Institute of Entomology, Nankai University for the identification of study species. This work was supported by the National Natural Science Foundation of China (31360154), the Project of Science and Technology Plan for Social Development of Yunnan Province (2012CH001), the Fund of Reserve Talents for Young and Middle-Aged Academic and Technological Leaders of Yunnan Province (2013HB077), and the Program for Innovative Research Team (in Science and Technology) in the University of Yunnan Province.

Author information

Authors and Affiliations

  1. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming, 650224, China

    Jia-Ying Zhu & Bin Yang

  2. Bureau of Forestry Pest Control and Quarantine of Yunnan Province, Kunming, 650051, China

    Sang-Zi Ze

  3. Beijing Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing, 100083, China

    Li-Jun Cao

Authors
  1. Jia-Ying Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sang-Zi Ze
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li-Jun Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bin Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sang-Zi Ze.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhu, JY., Ze, SZ., Cao, LJ. et al. Development of microsatellite markers for the plant bug, Pachypeltis micranthus (Hemiptera: Miridae). Appl Entomol Zool 51, 327–331 (2016). https://doi.org/10.1007/s13355-015-0392-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13355-015-0392-1

Keywords

Search

Navigation