Skip to main content
SpringerLink
Log in

Polymorphic microsatellite markers in the guava fruit fly, Bactrocera correcta (Diptera: Tephritidae)

  • Technical Note
  • Published:
Applied Entomology and Zoology Aims and scope Submit manuscript

Abstract

Bactrocera correcta (Bezzi) is one of the most destructive insect pests of fruits and vegetables in tropical and subtropical regions. At present, this fly is primarily distributed in Southeast Asia. Twelve microsatellite loci were isolated from an enriched genomic library based on a biotin/streptavidin capture protocol. The polymorphism of these loci was tested on 74 individual flies from two natural populations. Allele number ranged from 6 to 14 and 10 loci demonstrated a polymorphic information content (PIC) greater than 0.5. The pairwise F ST value between the two populations was 0.0048 (P > 0.05). These microsatellite loci have potential utility for studies of population genetic structure in this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aketarawong N, Bonizzoni M, Malacrida AR, Gasperi G, Thanaphum S (2006) Seventeen novel microsatellite markers from enriched library of the pest species Bactrocera dorsalis senso strict. Mol Ecol Notes 6:1138–1140

    Article  CAS  Google Scholar 

  • Augustinos AA, Stratikopoulus EE, Zacharopoulou A, Mathiopoulos KD (2002) Polymorphic microsatellite markers in the olive fly, Bactrocera oleae. Mol Ecol Notes 2:278–280

    Article  CAS  Google Scholar 

  • Botstein D, White RL, Skalnick MH, Davies RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am J Hum Genet 32:314–331

    PubMed  CAS  Google Scholar 

  • Brown J, Hardwick LJ, Wright AF (1995) A simple method for rapid isolation of microsatellites from yeast artificial chromosomes. Mol Cell Probes 9:53–57

    Article  PubMed  CAS  Google Scholar 

  • Dai SM, Lin CC, Chang C (2004) Polymorphic microsatellite DNA markers from the oriental fruit fly Bactrocera dorsalis (Hendel). Mol Ecol Notes 4:629–631

    Article  CAS  Google Scholar 

  • Dakin EE, Avise JC (2004) Microsatellite null alleles in parentage analysis. Heredity 93:504–509

    Article  PubMed  CAS  Google Scholar 

  • Drew RAI, Raghu S (2002) The fruit fly fauna (Diptera: Tephritidae: Dacinae) of the rainforest habitat of the Western Ghats, India. Raffles Bull Zool 50:327–352

    Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin version 3.0: an integrated software package for population genetic data analysis. Evol Bioinform Online 1:47–50

    CAS  Google Scholar 

  • Khamis F, Karam N, Guglielmino CR, Ekesi S, Masiga D, De Meyer M, Kenya EU, Malacrida AR (2008) Isolation and characterization of microsatellite markers in the newly discovered invasive fruit fly pest in Africa, Bactrocera invadens (Diptera: Tephritidae). Mol Ecol Res 8:1509–1511

    Article  CAS  Google Scholar 

  • Liang GQ, Yang GH, Liang F (1996) Insects of Bactrocera (Diptera: Tephritidae) in Asia-Pacific area. Guangdong Science and Technology Press, Guangdong, pp 58–59

    Google Scholar 

  • Liu JQ, Deng YL, Li ZH, Bai YH, Huang LG (2009) Advances in molecular biology of Bactrocera (Bactrocera) correcta. Plant Quarant 35:11–14 (in Chinese with English summary)

    Google Scholar 

  • Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655

    Article  PubMed  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP version 1.2: population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rozen S, Skaletsky HJ (2000) PRIMER 3.0 on the WWW for general users and for biologist programers. Methods Mol Biol 132:365–386

    PubMed  CAS  Google Scholar 

  • Shearman DCA, Gilchrist AS, Crisafulli D, Graham G, Lange C, Frommer M (2006) Microsatellite markers for the pest fruit fly, Bactrocera papaya (Diptera: Tephritidae) and other Bactrocera species. Mol Ecol Notes 6:4–7

    Article  CAS  Google Scholar 

  • Song SD, Ma J, Hughes JM (2006) Polymorphic microsatellite DNA markers in Bactrocera cacuminata (Hering) (Diptera: Tephritidae). Mol Ecol Notes 6:47–49

    Article  CAS  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • White IM, Elson-Harris MM (1992) Fruit flies of economic significance: their identification and bionomics. C.A.B. International and Australian Centre for International Agricultural Research, Wallingford

    Google Scholar 

  • Wu Y, Li ZH, Wu JJ (2009) Polymorphic microsatellite markers in the melon fruit fly, Bactrocera cucurbitae (Coquillet) (Diptera: Tephritidae). Mol Ecol Res 9:1404–1406

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank all other members of the Plant Quarantine Laboratory of China Agricultural University (CAUPQL). We certify that all authors have no financial or other conflict of interests in connection with the article. The experiments comply with the current laws of the People’s Republic of China. This study received financial support from the National Natural Science Foundation of China (No. 30971916) and the National Public Welfare Program (No. 201010042).

Author information

Authors and Affiliations

  1. Department of Entomology, College of Agriculture and Biotechnology, China Agricultural University, Yuanmingyuan West Road 2, Haidian District, Beijing, 100193, China

    Nopparat Buahom & Zhihong Li

  2. Yunnan Entry-Exit Inspection and Quarantine Bureau, No. 429, Dianchi Road, Kunming, 650228, Yunnan, China

    Yu Du & Xiaolong Jiang

  3. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Baodaoxincun, Danzhou, 571737, Hainan, China

    Yi Wu

  4. Xishuangbanna Entry-Exit Inspection and Quarantine Bureau, No. 65, Mengli Avenue, Jinghong, 666100, Xishuangbanna, Yunnan, China

    Yuliang Deng

  5. Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Huixinli No. 241, Huixinxijie, Chaoyang District, Beijing, 100029, China

    Wei Fu

Authors
  1. Nopparat Buahom
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuliang Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiaolong Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wei Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhihong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhihong Li.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Buahom, N., Du, Y., Wu, Y. et al. Polymorphic microsatellite markers in the guava fruit fly, Bactrocera correcta (Diptera: Tephritidae). Appl Entomol Zool 48, 409–412 (2013). https://doi.org/10.1007/s13355-013-0186-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13355-013-0186-2

Keywords

Search

Navigation