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Levofloxacin nanoemulsion gel has a powerful healing effect on infected wound in streptozotocin-induced diabetic rats

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Abstract

The aim of this study was to develop a novel oil-in-water (o/w) nanoemulsion gel containing levofloxacin for enhanced topical efficacy. Average particle size of sesame oil nanoemulsion without (SONE) and containing levofloxacin (SONEL) was found as 25.2 and 26.3 nm, respectively. Results from scratch test showed that SONEL had better proliferation effect in comparison with negative control. Treated animals with SONEL showed significant reduction in period of epithelialization, wound contraction, and number of inflammatory cells among all groups. Also, SONEL-treated group had the greatest collagen synthesis. Immunohistochemical analysis showed high intensity of CD31 and TGF-β at wound site of treatment groups with SONEL on day 12 post-treatment (P < 0.05). Skin irritation test demonstrated safety of SONEL gel for skin topical application. In conclusion, our studies suggest that SONEL could be an effective formulation for treatment of diabetic wound infection by controlling infection and improving the healing process.

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Abbreviations

SONE:

Sesame oil nanoemulsion

SONEL:

Sesame oil nanoemulsion containing levofloxacin

D-NC:

Diabetic negative control group

ND-NC:

Non-diabetic negative control group

SSD:

Silver sulfadiazine

CD31:

Cluster of differentiation 31

TGF-β:

Transforming growth factor beta

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Acknowledgments

This study was supported by Tehran University of Medical Science and Health Services grant no. 96-01-87-34142.

Author information

Authors and Affiliations

  1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 1417755469, Iran

    Alireza Valizadeh & Amir Amani

  2. Department of Microbiology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

    Mahdieh Shirzad

  3. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Pourmand

  4. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Farahmandfar

  5. School of Chemistry, College of Science, University of Tehran, Tehran, Iran

    Hassan Sereshti

  6. Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran

    Amir Amani

  7. Medical Biomaterials Research Center (MBRC), Tehran University of Medical Sciences, Tehran, Iran

    Amir Amani

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  1. Alireza Valizadeh
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  2. Mahdieh Shirzad
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  3. Mohammad Reza Pourmand
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Correspondence to Amir Amani.

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Valizadeh, A., Shirzad, M., Pourmand, M.R. et al. Levofloxacin nanoemulsion gel has a powerful healing effect on infected wound in streptozotocin-induced diabetic rats. Drug Deliv. and Transl. Res. 11, 292–304 (2021). https://doi.org/10.1007/s13346-020-00794-5

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