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Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay

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Abstract

Red sea bream iridovirus (RSIV) and Viral hemorrhagic septicemia virus (VHSV) are the most important viral marine pathogens in South Korea because RSIV and VHSV infect and cause high mortality rates in major fish species such as Paralichthys olivaceus and Sebastes schlegelii. These viruses can be transmitted both vertically and horizontally, and early diagnosis is imperative. In this research, RSIV and VHSV viral genomes are detected by PCR-lateral flow assay (LFA). PCR-LFA is sensitive, capable of detecting a viral genome at a concentration of 2–200 fg/µL. Development of this detection method is very meaningful because LFA is simple, requiring a minimum of personnel training to perform. Additionally, LFA requires less time than other detection methods and can be an immediate detection tool that is indispensable in preventing rapid viral spread.

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Acknowledgement

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (NRF-2017M3A9E407275).

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Author notes

  1. Eui-Joon Kil

    Present address: Department of Plant Medicals, Andong National University, Andong, 36729, Korea

Authors and Affiliations

  1. College of Biotechnology and Bioengineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, 16419, Korea

    Haneul Seo, Eui-Joon Kil, Chairina Fadhila, Thuy Thi Bich Vo & Sukchan Lee

  2. Department of Biological Science, Mokpo National University, Muan, 58554, Korea

    Chung-Kyoon Auh

  3. South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje, 53201, Korea

    Taek-Kyun Lee

Authors
  1. Haneul Seo
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  2. Eui-Joon Kil
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  3. Chairina Fadhila
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  4. Thuy Thi Bich Vo
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  5. Chung-Kyoon Auh
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  6. Taek-Kyun Lee
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  7. Sukchan Lee
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Correspondence to Sukchan Lee.

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Seo, H., Kil, EJ., Fadhila, C. et al. Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay. VirusDis. 31, 251–256 (2020). https://doi.org/10.1007/s13337-020-00577-z

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  • DOI: https://doi.org/10.1007/s13337-020-00577-z

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