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Differential expression of NADPH oxidase-2 (Nox-2) and nuclear factor-erythroid 2-related factor 2 (Nrf2) transcripts in peripheral blood mononuclear cells isolated from dengue patients

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Abstract

The role of oxidative stress in the pathogenesis of dengue infection is not completely known. A recent study reveals the involvement of oxidative stress responsive molecules in the generation of host immune responses to dengue virus in vitro. Objective of the present study was to analyse the changes in the expression of oxidant-antioxidant genes Nox-2 (NADPH oxidase) and Nrf2 (nuclear factor-erythroid 2-related factor 2) in patients with dengue during the early phase of infection compared to other febrile illness (OFI) cases and healthy controls using Real-time qPCR assay. The study enrolled 88 dengue patients, 31 OFI cases, and 63 healthy individuals as controls. Out of 88 dengue cases, 32 were classified as severe dengue cases (SD) and remaining 56 patients as non-severe dengue (NSD). Blood samples were collected firstly at the time of admission and a second sampling was done from the available individuals (38 dengue and 13 OFI cases) at the time of defervescence. Total RNA was extracted from the Peripheral blood mononuclear cells and the transcripts level of Nox-2 and Nrf2 were analysed by qPCR. On DOA, both Nox-2 and Nrf2 expression was found to be down regulated in dengue and OFI cases (P < 0.05) compared to healthy controls. Interestingly at defervescence, the transcript levels were found to be significantly increased in dengue cases unlike OFI, where no such increment was evidenced. From DOA to DOD, the study observed a signficant increase in the levels of Nox-2 transcripts (P < 0.05) both in SD and NSD cases. But a significant Nrf2 activation was not observed in SD cases as we found in NSD cases. Thus  a steady and significant increase in Nox-2 transcript level in severe, non-severe and secondary dengue infected groups observed in the current study supports the earlier reports on the involvement of anti-oxidant response in dengue severity. However further studies on its protein levels and mechanistic action would decipher the exact role of these potential molecules in the disease virulence.

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Acknowledgements

This work was supported by Indian council of medical research (ICMR) (Ref no: VIR/RCI/AES/58/2014/ECD-I) and intramural research grant from Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) (Ref no:JIP/Res/Intra-PhD/01/2014).

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Authors and Affiliations

  1. Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, 605 006, India

    Cleetus Cherupanakkal, Vijayakumar Ramachadrappa & Soundravally Rajendiran

  2. Department of Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India

    Tamilarasu Kadhiravan

  3. Department of Pediatrics, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India

    Narayanan Parameswaran

  4. Central Inter-Disciplinary Research Facility (CIDRF), A Unit of Sri Balaji Educational and Charitable Public Trust, Sri Balaji Vidyapeeth, MGMCRI Campus, Pondy-Cuddalore Mainroad, Pondicherry, India

    Agieshkumar Balakrishna Pillai

  5. Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India

    Subhash Chandra Parija

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  1. Cleetus Cherupanakkal
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  2. Vijayakumar Ramachadrappa
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  3. Tamilarasu Kadhiravan
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  5. Subhash Chandra Parija
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  6. Agieshkumar Balakrishna Pillai
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  7. Soundravally Rajendiran
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Correspondence to Agieshkumar Balakrishna Pillai or Soundravally Rajendiran.

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Cherupanakkal, C., Ramachadrappa, V., Kadhiravan, T. et al. Differential expression of NADPH oxidase-2 (Nox-2) and nuclear factor-erythroid 2-related factor 2 (Nrf2) transcripts in peripheral blood mononuclear cells isolated from dengue patients. VirusDis. 28, 54–60 (2017). https://doi.org/10.1007/s13337-017-0365-9

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  • DOI: https://doi.org/10.1007/s13337-017-0365-9

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