Abstract
Four samples of Plantago asiatica showing mottle or mottled yellowing symptoms were collected and three of them were confirmed to be infected with Plantago asiatica mosaic virus (PlAMV) by Illumina HiSeq 2500 paired-end RNA sequencing. Consequently, the complete genomic sequence of a Korean isolate of PlAMV (PlAMV isolate Gunwi) from P. asiatica was determined. The nucleotide sequence of PlAMV-Gunwi was most closely related to a Russian isolate of PlAMV (PlAMV-Ru) collected from P. asiatica but highly dissimilar (~23 %) to other isolates of PlAMV from other plant species. Pairwise comparisons revealed that the complete replicase protein and coat protein of PlAMV-Gunwi share 85.15–91.20 and 84.54–90.82 % amino acid sequence identities, respectively, to the corresponding proteins of other PlAMV isolates. To our knowledge, this is the first report of the natural infection of P. asiatica by PlAMV in Korea, and the divergent PlAMV isolate Gunwi expands our understanding of the epidemiology of PlAMV.
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References
Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M. Pfam: the protein families database. Nucl Acids Res. 2014;42:D222–30.
Hammond J, Bampi D, Reinsel MD. First report of Plantago asiatica mosaic virus in imported Asiatic and Oriental Lilies (Lilium hybrids) in the United States. Plant Dis. 2015;99(2):292.
Hughes PL, Harper F, Zimmerman MT, Scott SW. Nandina mosaic virus is an isolate of Plantago asiatica mosaic virus. Eur J Plant Pathol. 2005;113:309–13.
Kim EK, Kwon SB, Hong JS. Complete nucleotide sequence of Plantago Asiatica mosaic virus from Lilium spp. Korea Res Plant Dis. 2015;21(2):152.
Komatsu K, Yamaji Y, Ozeki J, Hashimoto M, Kagiwada S, Takahashi S, Namba S. Nucleotide sequence analysis of seven Japanese isolates of Plantago asiatica mosaic virus (PlAMV): a unique potexvirus with significantly high genomic and biological variability within the species. Arch Virol. 2008;153(1):193–8.
Lim S, Igori D, Yoo RH, Zhao F, Cho IS, Choi GS, Lim HS, Lee SH, Moon JS. Genomic detection and characterization of a Korean isolate of Little cherry virus 1 sampled from a peach tree. Virus Genes. 2015;51(2):260–6.
Lim S, Igori D, Zhao F, Yoo RH, An TJ, Lim HS, Lee SH, Moon JS. Complete genome sequence of a tentative new caulimovirus from the medicinal plant Atractylodes macrocephala. Arch Virol. 2015;160(12):3127–31.
Pájtli É, Eke S, Palkovics L. First report of the Plantago asiatica mosaic virus (PlAMV) Incidence on Lilium sp. in Hungary. Plant Dis. 2015;99(9):1288.
Parrella G, Greco B, Pasqualini A, Nappo AG. Plantago asiatica mosaic virus found in protected crops of Lily Hybrids in Southern Italy. Plant Dis. 2015;99(9):1289.
Solovyev AG, Novikov VK, Merits A, Savenkov EI, Zelenina DA, Tyulkina LG, Morozov SY. Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus. J Gen Virol. 1994;75:259–67.
Acknowledgments
This research was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01007703), Rural Development Administration, Republic of Korea.
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Lim, S., Igori, D., Zhao, F. et al. Molecular detection and characterization of a divergent isolate of Plantago asiatica mosaic virus in Plantago asiatica . VirusDis. 27, 307–310 (2016). https://doi.org/10.1007/s13337-016-0329-5
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DOI: https://doi.org/10.1007/s13337-016-0329-5