Abstract
Rice is an important agricultural crop providing nutrition for humans worldwide. Rice white tip nematode, Aphelenchoides besseyi, is a nematode causing economic yield losses globally in rice-growing areas. Because A. besseyi is a seed-transmitted nematode, its rapid and accurate identification is important for seed certification and quarantine purposes. Here, we developed species-specific primers for the small subunit ribosomal DNA region of the Aphelenchoides genus to identify A. besseyi. DNA was extracted from juveniles and directly infected tissues, such as hulls, brown rice and paddy rice. A. besseyi was easily and rapidly detected from plant tissues without nematode extraction. Because the extraction of A. besseyi from plant tissues is time-consuming and labour-intensive, the developed method may be applied to seed certification and quarantine approaches. To our knowledge, this is the first report directly identifying A. besseyi from infected plant tissues.
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Acknowledgments
This work was supported by The Scientific Research Projects Coordination Unit of Akdeniz University (Project No: FBA-2014-124). Elvan Sert Çelik from Akdeniz University for her DNA isolation assistance.
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Devran, Z., Tülek, A., Mıstanoğlu, İ. et al. A rapid molecular detection method for Aphelenchoides besseyi from rice tissues. Australasian Plant Pathol. 46, 43–48 (2017). https://doi.org/10.1007/s13313-016-0452-1
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DOI: https://doi.org/10.1007/s13313-016-0452-1