Abstract
Pitch canker is a disease that affects pines in many countries throughout the world. Fusarium circinatum, the causal agent of pitch canker, has recently been reported in Europe, where it is regulated as a quarantine pest. This pathogen represents a substantial threat to natural Pinus forests and plantations. To date, two mating types of F. circinatum have been described, both of which are present in Spain. To assess the potential risk of F. circinatum to Pinus in Spain, the main conifer species grown in Spain, namely Pinus sylvestris , P. nigra, P. pinaster, P. radiata , P. halepensis P. pinea and P. uncinata , were examined for their susceptibility to four Spanish isolates of F. circinatum. Two-year-old shoots were wounded and then inoculated with the four Spanish isolates, and lesion lengths were measured 2 weeks after inoculation. Pinus radiata was found to be the most susceptible species to the Spanish isolates, whereas P. pinea, P. halepensis, P. nigra, P. uncinata and P. pinaster were found to be relatively resistant and showed a significantly different lesion size from P. radiata. For P. pinaster, variations in resistance were found between provenances. One particular provenance (Meseta Castellana) was found to be more resistant than the three other provenances tested. No differences in virulence between the Mat-1 and Mat-2 isolates were detected. Overall, the low susceptibility of all native species to pitch canker suggests a moderate potential risk to the Spanish natural forests and a high potential risk to the exotic forestry of P. radiata.
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References
Aegerter BJ, Gordon TR (2006) Rates of pitch canker induced seedling mortality among Pinus radiata families varying in levels of genetic resistance to Gibberella circinata (anamorph Fusarium circinatum). Forest Ecol Manage 235:14–17
Braganca H, Diogo E, Moniz F, Amaro P (2009) First report of pitch canker on pines caused by Fusarium circinatum in Portugal. Plant Dis 93:1079
Carlucci A, Colatruglio L, Frisullo S (2007) First report of pitch canker caused by Fusarium circinatum on Pinus halepensis and P. pinea in Apulia (southern Italy). Plant Dis 91:1683
DGCONA (1998) Segundo Inventario Forestal Nacional, IFN-II, 1986–1996, España. Ministerio de de Medio Ambiente, Madrid
Enebak SA, Stanosz GR (2003) Responses of conifer species of the Great Lakes region of North America to inoculation with the pitch canker pathogen Fusarium circinatum. For Pathol 33:333–338
European and Mediterranean Plant Protection Organization (2005) EPPO data sheets on quarantine pests-Giberella circinata. Bulletin OEPP/EPPO 35:383–386
European and Mediterranean Plant Protection Organization (2011) EPPO plant quarantine data retrieval system (PQR Version 5.0)
Ganley RJ, Watt MS, Lucy M, Iturritxa E (2009) A global climatic risk assessment of pitch canker disease. Can J For Res 39(11):2246–2256
Gordon TR, Okamoto D, Storer AJ, Wood DL (1998a) Susceptibility of five landscape pines to pitch canker disease, caused by Fusarium subglutinans f. sp. pini. Hortscience 33:868–871
Gordon TR, Wikler KR, Clark L, Okamoto D, Storer AJ, Bonello P (1998b) Resistance to pitch canker disease, caused by Fusarium subglutinans f. sp. pini, in Monterey pine (Pinus radiata). Plant Pathol 47:706–711
Gordon TR, Storer AJ, Wood DL (2001) The pitch canker epidemic in California. Plant Dis 85:1128–1139
Gordon TR, Kirkpatrick SC, Aegerter BJ, Wood DL, Storer AJ (2006) Susceptibility of Douglas fir (Pseudotsuga menziesii) to pitch canker, caused by Gibberella circinata (anamorph = Fusarium circinatum). Plant Pathol 55:231–237
Hepting GH, Roth ER (1946) Pitch canker, a new disease of some southern pines. J For 44:724–744
Hodge GR, Dvorak WS (2000) Differential responses of Central American and Mexican pine species and Pinus radiata to infection by the pitch canker fungus. New Forests 19:241–258
Iturritxa E, Ganley RJ, Wright J, Heppe E, Steenkamp T, Gordon TR, Wingfield M (2011) A genetically homogenous population of Fusarium circinatum causes pitch canker of Pinus radiata in the Basque Country, Spain. Fungal Biology 115:288–295
Kayihan GC, Huber DA, Morse AM, White TL, Davis JM (2005) Genetic dissection of fusiform rust and pitch canker disease traits in loblolly pine. Theor Appl Genet 110:948–958
Kim Y-S, Woo K-S, Koo Y-B, Yeo J-K (2008) Variation in susceptibility of six pine species and hybrids to pitch canker caused by Fusarium circinatum. For Pathol 38:419–428
Kobayashi T, Muramoto M (1989) Pitch canker of Pinus luchuensis, a new disease of Japanese forests. Forest Pests 40:169–173
Landeras E, García P, Fernández Y, Braña M, Fernández-Alonso O, Méndez-Lodos S, Pérez-Sierra A, León M, Abad-Campos P, Berbegal M, Beltrán R, García-Jiménez J, Armengol J (2005) Outbreak of Pitch Canker Caused by Fusarium circinatum on Pinus spp. in Northern Spain. Plant Dis 89:1015
Nirenberg HI, O’Donnell K (1998) New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434–458
Pérez-Sierra A, Landeras E, León M, Berbegal M, García-Jiménez J, Armengol J (2007) Characterization of Fusarium circinatum from Pinus spp. in northern Spain. Mycol Res 111:832–839
Roux J, Eisenberg B, Kanzler A, Nel A, Coetzee Y, Kietzka E, Wingfield MJ (2007) Testing of selected South African Pinus hybrids and families for tolerance to the pitch canker pathogen, Fusarium circinatum. New Forests 33:109–123
Serrada R, Montero M, Reque, J (2008) Compendio de Selvicultura Aplicada en España. INIA y FUCOVASA. Madrid. ISBN.: 978-84-7498-521-4, pp 1.178
Sokal RR, Rohlf FJ (1995) Biometry, 3rd ed. Ed. Freeman
Storer AJ, Bonello P, Gordon TR, Wood DL (1999) Evidence of resistance to the pitch canker pathogen (Fusarium circinatum) in native stands of Monterey pine (Pinus monticola). Forest Sci 45:500–505
Viljoen A, Wingfield MJ, Marasas WFO (1994) First report of Fusarium subglutinans f. sp. pini on seedlings in South Africa. Plant Dis 78:309–312
Viljoen A, Wingfield MJ, Kemp GHJ, Marasas WFO (1995) Susceptibility of pines in South Africa to the pitch canker fungus Fusarium subglutinans f. sp. Pini. Plant Pathol 44:877–882. doi:10.1111/365-3059,1995.tb02747
Wingfield MJ, Jacobs A, Coutinho TA, Ahumada R, Wingfield BD (2002) First report of the pitch canker fungus, Fusarium circinatum, on pines in Chile. Plant Pathol 51:397. doi:j./j.1365-3059,2002.00710
Wingfield MJ, Hammerbacher A, Ganley RJ, Steenkamp ET, Gordon TR, Wingfield BD, Coutinho TA (2008) Pitch canker caused by Fusarium circinatum-a growing threat to pine plantations and forests worldwide. Australas Plant Pathol 37:319–334
Acknowledgements
We thank the INVITROTEK and GONDRA nurseries for providing plant material from different species and provenances.
This research was supported in part by Project AEG08-008 (joint program INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria and Ministerio de Medio Ambiente, Rural y Marino, Spain); and DAPA, Agriculture and Environment Department of the Basque Government. Y. Serrano was supported by Project AEG08-008, and N. Mesanza was supported by the University and Research Department of the Basque Government.
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Iturritxa, E., Mesanza, N., Elvira-Recuenco, M. et al. Evaluation of genetic resistance in Pinus to pitch canker in Spain. Australasian Plant Pathol. 41, 601–607 (2012). https://doi.org/10.1007/s13313-012-0160-4
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DOI: https://doi.org/10.1007/s13313-012-0160-4