Skip to main content
SpringerLink
Log in

Efficacy of fungicides on mycelial growth of diatrypaceous fungi associated with grapevine trunk disease

  • Published:
Australasian Plant Pathology Aims and scope Submit manuscript

Abstract

Several species of Diatrypaceae have been recently isolated from the wood of cankered grapevines in several regions of the world and shown to be pathogenic with varying degrees of virulence when inoculated in stems of potted vines. Existing management strategies have focussed on the development of procedures and products to prevent or reduce Eutypa lata infection. The best method to prevent infection is by applying fungicides to wounds, but there are few registered chemicals for any of the diatrypaceous fungi in Australia. Six selected fungicides were evaluated in vitro for their efficacy in reducing mycelial growth of Eutypa lata, Cryptovalsa ampelina, Diatrypella vulgaris, Eutypa leptoplaca, Eutypella citricola and Eutypella microtheca. Carbendazim, fluazinam, tebuconazole, and prothioconazole + tebuconazole were effective at inhibiting mycelial growth of all Diatrypaceae spp. tested. Pyraclostrobin reduced colony diameter of most of the fungal species by 50% or more. Pyrimethanil was ineffective at reducing mycelial growth of these pathogens. Five of the six fungicides evaluated in this study have shown efficacy in vitro against Diatrypaceae spp. and require further evaluation in the field. This study represents the first approach for fungicide evaluation against mycelial growth of diatrypaceous fungi, other than Ea. lata. It contributes to the development of integrated management strategies for grapevine trunk diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Bourbos VA, Barbopoulou EA (2005) Study of the possibility to control Eutypa lata (Pers. Fr.) Tul. in grapevine. Phytopathol Mediterr 44:116

    Google Scholar 

  • Carter MV (1978) Eutypa dieback (“Dying Arm”) disease of vines—progress towards control. Aust Grapegrower Winemaker 172:27–28

    Google Scholar 

  • Carter MV (1991) The status of Eutypa lata as a pathogen. Monograph—Phytopathological Paper 32. International Mycological Institute: Surrey, UK

  • Carter MV, Price TV (1975) Biological control of Eutypa armeniacae. III. A comparison of chemical, biological and integrated control. Aust J Agric Res 26:537–543

    Article  CAS  Google Scholar 

  • Catal M, Jordan SA, Butterworth SC, Shilder AMC (2007) Detection of Eutypa lata and Eutypella vitis in grapevine by nested multiplex polymerase chain reaction. Phytopathology 97:737–747

    Article  PubMed  CAS  Google Scholar 

  • Díaz GA, Prehn D, Latorre BA (2011) First report of Cryptovalsa ampelina and Eutypella leprosa associated with grapevine trunk diseases in Chile. Plant Dis 95:490

    Article  Google Scholar 

  • Ferreira JHS, Matthee FN, Thomas AC (1991) Biological control of Eutypa lata on grapevine by an antagonistic strain of Bacillus subtilis. Phytopathology 81:283–287

    Article  Google Scholar 

  • Gendloff EH, Ramsdell DC, Burton CL (1983) Fungicidal control of Eutypa armeniacae infecting Concord grapevine in Michigan. Plant Dis 67:754–756

    Article  CAS  Google Scholar 

  • Halleen F, Fourie PH (2005) Protection of grapevine pruning wounds against fungal infections. Phytopathol Mediterr 44:117–118

    Google Scholar 

  • Halleen F, Fourie PH, Calitz FJ (2001) In vitro sensitivity of Eutypa lata to various fungicides. Wynboer August, 113–115.

  • Halleen F, Fourie PH, Lombard PJ (2010) Protection of grapevine pruning wounds against Eutypa lata by biological and chemical methods. S Afr J Enol Viticult 31:125–132

    CAS  Google Scholar 

  • John S, Wicks TJ, Hunt JS, Lorimer MF, Oakey H, Scott ES (2005) Protection of grapevine pruning wounds from infection by Eutypa lata using Trichoderma harzianum and Fusarium lateritium. Australas Plant Pathol 34:569–575

    Article  Google Scholar 

  • Lecomte P, Laveau E, Laterriere SG, Dewasme C, Clerjeau M (2003) Optimization of pruning wound protection for the control of Eutypa dieback of grapevine in France. Proceedings of the IOBC WPRS working group ‘Integrated Protection and Production in Viticulture’, Volos, Greece, pp. 95–96

  • Loschiavo AP, Sosnowski MR, Wicks TJ (2007) In vitro screening of treatments to control eutypa dieback in grapevines. 16th Australasian Plant Pathology Conference, Adelaide 24–27 September 2007 p142

  • Luque J, Sierra D, Torres E, Garcia F (2006) Cryptovalsa ampelina on grapevines in N.E. Spain: identification and pathogenicity. Phytopathol Mediterr 45:S101–S109

    Google Scholar 

  • McMahan G, Yeh W, Marshall MN, Olsen M, Sananikone S, Wu JY, Block DE, VanderGheynst JS (2001) Characterizing the production of a wild-type and benomyl- resistant Fusarium lateritium for biocontrol of Eutypa lata on grapevine. J Ind Microb Biotech 26:151–155

    Article  CAS  Google Scholar 

  • Moller WJ, Kasimatis AN (1980) Protection of grapevine pruning wounds from Eutypa dieback. Plant Dis 64:278–280

    Article  Google Scholar 

  • Mostert L, Halleen F, Creaser ML, Crous PW (2004) Cryptovalsa ampelina, a forgotten shoot and cane pathogen of grapevines. Australas Plant Pathol 33:295–299

    Article  Google Scholar 

  • Munkvold GP, Marois JJ (1993a) The effects of fungicides on Eutypa lata germination, growth, and infection of grapevines. Plant Dis 77:50–55

    Article  CAS  Google Scholar 

  • Munkvold GP, Marois JJ (1993b) Efficacy of natural epiphytes and colonisers of grapevine pruning wounds for biological control of Eutypa dieback. Phytopathology 83:624–629

    Article  Google Scholar 

  • Pearson RC (1982) Protection of grapevine pruning wounds from infection by Eutypa armeniacae in New York State. Am J Enol Viticult 33:51–52

    CAS  Google Scholar 

  • Petzoldt CH, Moller WJ, Sall MA (1981) Eutypa dieback of grapevine: seasonal differences in infection and duration of susceptibility of pruning wounds. Phytopathology 71:540–543

    Article  Google Scholar 

  • Phillips AJL (2002) Botryosphaeria species associated with diseases of grapevines in Portugal. Phytopathol Mediterr 41:3–18

    Google Scholar 

  • Pitt WM, Huang R, Trouillas FP, Steel CC, Savocchia S (2010) Evidence that Eutypa lata and other diatrypaceous species occur in New South Wales vineyards. Australas Plant Pathol 39:97–106

    Article  Google Scholar 

  • Ramsdell DC (1995) Winter air-blast sprayer applications of benomyl for reduction of Eutypa dieback disease incidence in a concord grape vineyard in Michigan. Plant Dis 79:399–402

    Article  Google Scholar 

  • Rolshausen PE, Gubler WD (2005) Use of boron for the control of Eutypa dieback of grapevines. Plant Dis 89:734–738

    Article  CAS  Google Scholar 

  • Rolshausen PE, Mahoney NE, Molyneux RJ, Gubler WD (2006) A re-assessment of the species concept in Eutypa lata, the causal agent of Eutypa dieback of grapevine. Phytopathology 96:369–377

    Article  PubMed  CAS  Google Scholar 

  • Rolshausen PE, Úrbez-Torres JR, Rooney-Latham S, Eskalen A, Smith RJ, Gubler WD (2010) Evaluation of pruning wound susceptibility and protection against fungi associated with grapevine trunk diseases. Am J Enol Viticult 61:113–119

    Google Scholar 

  • Safodien S (2007) The molecular identification and characterization of Eutypa die-back and a PCR-based assay for the detection of Eutypa and Botryosphaeriaceae species from grapevine in South Africa. Master’s thesis, University of Stellenbosch

  • Siebert JB (2001) Eutypa: The economic toll on vineyards. Wines and Vines April, 50–56

  • Sosnowski MR, Creaser M, Wicks TJ (2004) Managing eutypa dieback of grapevines by remedial surgery. Aust NZ Grapegrower Winemaker 488:35–39

    Google Scholar 

  • Sosnowski MR, Lardner R, Wicks TJ, Scott ES (2007) The influence of grapevine cultivar and isolate of Eutypa lata on wood and foliar symptoms. Plant Dis 91:924–931

    Article  Google Scholar 

  • Sosnowski MR, Creaser M, Wicks TJ, Lardner R, Scott ES (2008) Protection of grapevine pruning wounds from infection by Eutypa lata. Aust J Grape Wine Res 14:134–142

    Article  Google Scholar 

  • Tey-Rulh P, Philippe I, Renaud JM, Tsoupras G, De Angelis P, Fallot J, Tabacchi R (1991) Eutypine, a phytotoxin produced by Eutypa lata the causal agent of dying-arm disease of grapevine. Phytochemistry 30:471–473

    Article  CAS  Google Scholar 

  • Trouillas FP, Gubler WD (2004) Identification and characterization of Eutypa leptoplaca, a new pathogen of grapevine in Northern California. Myc Res 108:1195–1204

    Article  CAS  Google Scholar 

  • Trouillas FP, Úrbez-Torres JR, Gubler WD (2010) Diversity of diatrypaceous fungi associated with grapevine canker diseases in California. Mycologia 102:319–336

    Article  PubMed  CAS  Google Scholar 

  • Trouillas FP, Pitt WM, Sosnowski MR, Huang R, Peduto F, Loschiavo A, Savocchia S, Scott E, Gubler WD (2011) Taxonomy and DNA phylogeny of Diatrypaceae associated with Vinis vinifera and other woody plants in Australia. Fungal Divers 49:203–223

    Article  Google Scholar 

  • Úrbez-Torres JR, Leavitt GM, Voegel T, Gubler WD (2006) Identification and distribution of Botryosphaeria species associated with grapevines cankers in California. Plant Dis 90:1490–1503

    Article  Google Scholar 

  • Úrbez-Torres JR, Adams P, Kamas J, Gubler WD (2009) Identification, incidence, and pathogenicity of fungal species associated with grapevine dieback in Texas. Am J Enol Viticult 60:497–507

    Google Scholar 

  • Wicks T, Davies K (1999) The effect of Eutypa on grapevine yield. Aust Grapegrower Winemaker 426a:15–16

    Google Scholar 

Download references

Acknowledgements

We thank Dr. Josep Armengol for critically reading the manuscript prior to submission. Financial support by the ‘Programa de Apoyo a la Investigación y Desarrollo (PAID-00-10) de la Universidad Politécnica de Valencia’ for David Gramaje during his 3 months in Australia is gratefully acknowledged. This research was also supported by Australia’s grapegrowers and winemakers through their investment body, the Grape and Wine Research and Development Corporation, with matching funds from the federal government.

Author information

Author notes

  1. David Gramaje

    Present address: Institute of Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Alameda del Obispo s/n, P.O. Box 4084, 14080, Córdoba, Spain

Authors and Affiliations

  1. Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain

    David Gramaje

  2. South Australian Research and Development Institute, Adelaide, SA, 5001, Australia

    Matthew R. Ayres & Mark R. Sosnowski

  3. Department of Plant Pathology, University of California, Davis, CA, 95616, USA

    Florent P. Trouillas

Authors
  1. David Gramaje
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Matthew R. Ayres
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Florent P. Trouillas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark R. Sosnowski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mark R. Sosnowski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gramaje, D., Ayres, M.R., Trouillas, F.P. et al. Efficacy of fungicides on mycelial growth of diatrypaceous fungi associated with grapevine trunk disease. Australasian Plant Pathol. 41, 295–300 (2012). https://doi.org/10.1007/s13313-011-0111-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13313-011-0111-5

Keywords

Search

Navigation