Abstract
Previous studies verified that miR-214 is of great significance in the invasion and migration of a variety of cancers. It has been demonstrated that UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7(GALNT7) is a putative target of miR-214. We performed this study to figure out how miR-214 and GALNT7 play their roles in the invasion and migration of esophageal squamous cell carcinoma (ESCC). The expression of miR-214 was significantly downregulated in tumors compared to the corresponding non-tumor tissues while GALNT7 showed an opposite tendency. The low expression of miR-214 and the high expression of GALNT7 were found positively correlated with poor tumor differentiation (P = 0.004), tumor invasion (P = 0.013), and lymph node metastasis (P = 0.012) in ESCC patients. Functional study demonstrated that overexpression of miR-214 or knockdown of GALNT7 could weaken invasive and migratory ability in Eca109, TE1, and KYSE150. Moreover, tumorigenicity assay showed us mice injected with cells containing miR-214 mimic or GALNT7 small interfering RNA formed substantially smaller tumors than that in miR-214 inhibitor group. Consequently, we concluded that miR-214 shows potential to be a diagnostic marker and therapeutic target in ESCC.
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Our study was supported by the National Natural Science Foundation of China (grant number 81472688, 81301829).
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Approved by the Medical Ethics Committee of Changhai Hospital, tumor specimens and corresponding adjacent normal tissue specimens, at least 5 cm far from the tumor lesion margin, were collected from consenting patients.
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Qijue Lu, Li Xu, and Chunguang Li contributed equally to this work.
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Lu, Q., Xu, L., Li, C. et al. miR-214 inhibits invasion and migration via downregulating GALNT7 in esophageal squamous cell cancer. Tumor Biol. 37, 14605–14614 (2016). https://doi.org/10.1007/s13277-016-5320-7
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DOI: https://doi.org/10.1007/s13277-016-5320-7