Abstract
Uveal melanoma (UM) has a high mortality rate for primary intraocular tumors. Approximately half of UM patients present with untreatable and fatal metastases. Long non-coding RNAs (lncRNAs) have emerged as potent regulatory RNAs that play key roles in various cellular processes and tumorigenesis. However, to date, their roles in UM are not well-known. Here, we identified a transcriptional variant transcribed from the P2RX7 gene locus, named P2RX7-V3 (P2RX7 variant 3), which was expressed at a high level in UM cells. P2RX7-V3 silencing revealed that this variant acts as a necessary UM oncoRNA. Knockdown of P2RX7-V3 expression significantly suppressed tumor growth in vitro and in vivo. A genome-wide cDNA array revealed that a variety of genes were dysregulated following P2RX7-V3 silencing. These observations identified P2RX7-V3 that plays a crucial role in UM tumorigenesis and may serve as a useful biomarker in the diagnosis and prognosis treatment of UM in the future.
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Acknowledgments
This work was supported by Scientific Research Program of The National Health and Family Planning Commission of China (201402014) for XQF, The National Natural Science Foundation of China (grant 31470757), The Program for Professor of Special Appointment (Eastern Scholar) at the Shanghai Institutions of Higher Learning (1410000159), The Shanghai PuJiang Program (13PJ1405700), SMC-ChenXing Yong Scholar Program (2014, Class B), and The Science and Technology Commission of Shanghai (grants 14JC1404100, 14JC1404200, 14430723100). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Hui Pan and Hongyan Ni contributed equally to this work
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Pan, H., Ni, H., Zhang, L. et al. P2RX7-V3 is a novel oncogene that promotes tumorigenesis in uveal melanoma. Tumor Biol. 37, 13533–13543 (2016). https://doi.org/10.1007/s13277-016-5141-8
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DOI: https://doi.org/10.1007/s13277-016-5141-8