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MicroRNA-101 polymorphisms and risk of head and neck squamous cell carcinoma in a Chinese population

  • Original Article
  • Published:
Tumor Biology

Abstract

MicroRNAs (miRNAs) play important roles in regulation of gene expressions and likely have involvement in cancer susceptibility and disease progression. MicroRNA-101 (miR-101) has been well established as a tumor suppressor, and aberrant expression of miR-101 levels has been previously reported in different malignancies including head and neck squamous cell carcinoma (HNSCC). However, the role of single nucleotide polymorphisms (SNPs) of miR-101 in the susceptibility to HNSCC remains unclear. In this study, we genotyped 11 selected SNPs of the miR-101 genes (including miR-101-1 and miR-101-2) in a case–control study including 576 HNSCC cases and 1552 cancer-free controls. For the main effect analysis, none of the 11 selected SNPs was associated with HNSCC risk. However, in the stratification analysis by tumor sites, rs578481 and rs705509 in pri-miR-101-1 were significantly associated with risk of oral squamous cell carcinoma (OSCC) (rs578481: adjusted odds ratio (OR) = 1.19, 95 % confidence interval (CI) 1.01–1.39, P = 0.036; rs705509: adjusted OR = 0.85, 95 % CI 0.73–0.98, P = 0.030). Furthermore, combined analysis of the two SNPs revealed that subjects carrying the risk alleles of rs578481 and rs705509 had increased risk of OSCC in a dose–response manner (P trend = 0.022). Compared with subjects carrying “0–2” risk alleles, subjects carrying “3–4” risk alleles presented a 1.38-fold increased risk of OSCC. In conclusion, our findings suggested that the SNPs rs578481 and rs705509 locating in pri-miR-101-1 may play a role in genetic susceptibility to OSCC, which may improve our understanding of the potential contribution of miRNA SNPs to cancer pathogenesis.

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Abbreviations

HNC:

Head and neck cancer

HNSCC:

Head and neck squamous cell carcinoma

OSCC:

Oral squamous cell carcinoma

UTR:

Untranslated region

SNPs:

Single nucleotide polymorphisms

OR:

Odds ratio

CI:

Confidence interval

ESCC:

Esophageal squamous cell carcinoma

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Acknowledgments

This work was supported in part by the National Natural Science Foundation of China (81473048 and 81302361), Priority Academic Program Development of Jiangsu Higher Education Institutions (Public Health and Preventive Medicine), Specialized Research Fund for the Doctoral Program of Higher Education of China (20133234120013), China Postdoctoral Science Foundation (2013 M540457), and Jiangsu Planned Projects for Postdoctoral Research Funds (1301018A).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 210029, China

    Limin Miao, Hua Yuan, Longbiao Zhu, Bing Li, Ruixia Wang & Ning Chen

  2. Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, 211166, China

    Lihua Wang, Dong Hang, Jiangbo Du, Xun Zhu & Hongxia Ma

  3. Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, 211166, China

    Hongxia Ma

  4. Institute of Stomatology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, China

    Ning Chen

Authors
  1. Limin Miao
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  2. Lihua Wang
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  3. Hua Yuan
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  4. Dong Hang
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  5. Longbiao Zhu
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  6. Jiangbo Du
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  7. Xun Zhu
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  8. Bing Li
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  9. Ruixia Wang
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  10. Hongxia Ma
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  11. Ning Chen
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Corresponding authors

Correspondence to Hongxia Ma or Ning Chen.

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Miao, L., Wang, L., Yuan, H. et al. MicroRNA-101 polymorphisms and risk of head and neck squamous cell carcinoma in a Chinese population. Tumor Biol. 37, 4169–4174 (2016). https://doi.org/10.1007/s13277-015-4249-6

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  • DOI: https://doi.org/10.1007/s13277-015-4249-6

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