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Combination of miRNA and RNA functions as potential biomarkers for gastric cancer

  • Research Article
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Tumor Biology

Abstract

Gastric cancer (GC) is the second leading cause of cancer-related death in the world. The optimal treatment regimens for GC depend on tumor stage, histopathological subtype, and other factors. The detection of tumor biomarkers is a quick way to get information of the tumor state. In this study, new biomarkers are detected for GC diagnostic and prognostic purposes. A total of 305 cases of diagnosed gastric adenocarcinoma were enrolled, microRNAs (miRNAs) and their transcriptome sequencing data were obtained from the “The Cancer Genome Atlas.” Blood samples were collected from GC patients before surgery and therapy. The miRNA levels and the expression of RNA were detected by real-time RT-PCR. Receiver operating characteristic analysis was used to evaluate the sensitivity and specificity of biomarkers. The combining predictors were established with the logistic regression analysis. Hundreds of miRNA were with higher area under curve (AUC) than 0.5; among them, nine miRNAs were with the highest AUC more than 0.90 and displayed strong diagnostic value. Moreover, the mir-17 level was correlated with tumor stage (p = 0.029), while mir-133b, mir-133a-2, and mir-1-2 levels were significantly correlated with race, tumor pathologic, and tumor stage (p < 0.05). The combination biomarker (mir-181a-1/KAT2B with a sensitivity of 95.83 % and specificity of 94.12 %) could be used as an independent diagnostic indicator for GC patients. For GC patients, mir-17, mir-133b, mir-133a-2, and mir-1-2 appear to be a potential novel predictor of tumor stage and preoperative and intraoperative diagnosis. The combination of miRNA and mRNA such as mir-181a-1/KAT2B (with a sensitivity of 95.83 % and specificity of 94.12 %) showed significant improvement in the diagnostic accuracy.

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Acknowledgments

Thanks are given to Lizann Oswald for the revision of this manuscript. This study was supported by NSFC grant (30770649, 30970682, 31370837), Research Fund for Science and Technology Program of Jilin Province (20150101142JC), Ministry of Health of Jilin Province (20142036), Health and Family Planning Commission of Jilin Province (20142036), and Natural Science Foundation of Jilin Province (20150101142JC).

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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    Authors and Affiliations

    1. Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, 130021, China

      Silin Chen, Feifei Yu, Yuxi Tian, Shumei Ma & Xiaodong Liu

    2. 2nd Hospital Jilin University, Changchun, 130041, China

      Jiaming Zhu

    3. Institute of Cancer Care, University of Manitoba, Winnipeg, MB, R3T 3T2, Canada

      Shumei Ma

    4. Center for Radiological Research, Columbia University, New York, NY, 10032, USA

      Xiaodong Liu

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    1. Silin Chen
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    2. Jiaming Zhu
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    3. Feifei Yu
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    Correspondence to Shumei Ma.

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    Silin Chen and Jiaming Zhu equally contributed to this study.

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    Chen, S., Zhu, J., Yu, F. et al. Combination of miRNA and RNA functions as potential biomarkers for gastric cancer. Tumor Biol. 36, 9909–9918 (2015). https://doi.org/10.1007/s13277-015-3756-9

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    • DOI: https://doi.org/10.1007/s13277-015-3756-9

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