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A Huaier polysaccharide reduced metastasis of human hepatocellular carcinoma SMMC-7721 cells via modulating AUF-1 signaling pathway

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Tumor Biology

Abstract

TP-1 is a polysaccharide from one famous fungus Huaier. Treatment with TP-1 significantly inhibited the cell growth, adhesion, migration, and motility of SMMC-7721 cells in a dose-dependent manner. Real-time quantitative RT-PCR revealed a dose-dependent decrease in RNA-binding factor 1 (AUF-1) and astrocyte elevated gene-1 (AEG-1) messenger RNA (mRNA) levels in TP-1-treated SMMC-7721 cells, which is consistent with their protein expression detected by Western blotting. On the contrary, microRNA-122 (miR-122) expression increased in SMMC-7721 cells following TP-1 treatment. Moreover, TP-1 treatment at three doses apparently increased epithelial marker E-cadherin protein expression but decreased the mesenchymal marker N-cadherin protein level. In addition, the hematoxylin-eosin (H & E) staining showed that the TP-1 significantly inhibited the lung metastasis of liver cancer in mice orthotopic implanted with SMMC-7721 tumor tissue. Taken together, these findings proved the inhibitory effect of TP-1 on the growth and metastasis of SMMC-7721 cells, and TP-1 might be offered for future application as a powerful chemopreventive agent against hepatocellular carcinoma (HCC) metastasis.

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Acknowledgments

This research is funded by the National Natural Science Foundation of China (grant no. 81472328); Liver Disease AIDS Foundation of You An Hospital, Capital Medical University (BJYAH-2011-034); National Science and Technology Support Project (2012BAI15B08); and Key Project of National Communicable Disease (2012ZX10002015-002).

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The authors declare that they have no conflicts of interest concerning this article.

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    Authors and Affiliations

    1. Intervention Therapy Center of Liver Diseases, Beijing You An Hospital, Capital Medical University, 100069, Beijing, China

      Cong Li, Honghai Zhang, Gengxia Yang, Meijun Hao, Shoupeng Sheng, Yu Sun, Jiang Long, Caixia Hu & Jiasheng Zheng

    2. Department of Infectious Disease, the Second Affiliated Hospital of Harbin Medical University, 150081, Harbin, China

      Xia Wu

    3. School of Medicine, Tsinghua Center for Life Sciences, Tsinghua University, 100084, Beijing, China

      Xicai Sun

    4. Institute of Liver Diseases, Beijing You An Hospital, Capital Medical University, 100069, Beijing, China

      Li Li

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    1. Cong Li
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    Correspondence to Jiasheng Zheng.

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    Cong Li and Xia Wu contributed equally to this work.

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    Li, C., Wu, X., Zhang, H. et al. A Huaier polysaccharide reduced metastasis of human hepatocellular carcinoma SMMC-7721 cells via modulating AUF-1 signaling pathway. Tumor Biol. 36, 6285–6293 (2015). https://doi.org/10.1007/s13277-015-3314-5

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