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Role of survivin re-expression in the development and progression of non-small cell lung cancer

  • Research Article
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Tumor Biology

Abstract

Survivin is highly expressed in fetal tissue and is completely absent in terminally differentiated cells, but its re-expression has been observed in most human tumors. Presently, we aimed to analyze the possible impact of the survivin gene (−31G > C, rs 9904341) promoter polymorphism on the expression profile of survivin gene and ultimately the role of survivin re-expression in the development and progression of non-small cell lung cancer. A case-control study of 100 non-small cell lung cancer patients and 100 cancer-free healthy controls was conducted. Survivin gene promoter polymorphism was analyzed by PCR-restriction fragment length polymorphisms (RFLP) technique, and the survivin expression profile was evaluated using quantitative real-time PCR assay. Compared to the survivin GG genotype, odd ratio of 3.2 (95 % CI 4.8–25.9, p = 0.004) was found to be associated to homozygous CC genotype with 15-fold increase of survivin gene expression in non-small cell lung cancer patients. Significant trend of increase in survivin expression was observed with the increase in severity of the disease. Patients with survivin (−31CC) genotype had significantly shorter overall survival compared to survivin (−31GG) genotype carriers. In addition, advanced disease status and significant poor overall survival were also reflected by patients with higher-fold increase in survivin gene expression. In conclusion, present study demonstrated that survivin (−31G > C) polymorphism may contribute to the risk of developing non-small cell lung cancer in Indian population. Survivin (−31CC) genotype was associated with significantly increased survivin gene expression and ultimately may contribute in the poor clinical outcome of non-small cell lung cancer patients, suggesting its possible significance in the development and progression of non-small cell lung cancer.

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Abbreviations

NSCLC:

Non-small cell lung cancer

SCC:

Squamous cell carcinoma

ADC:

Adenocarcinoma

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Acknowledgments

The authors specially thank all of the patients who participated in this study.

Conflicts of interest

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Authors and Affiliations

  1. Cancer Genetics Lab, Department of Biochemistry, Maulana Azad Medical College and Associated hospitals, New Delhi, India

    Jamsheed Javid

  2. Division of Molecular Genetics, Prince Fahd Bin Sultan Research Chair, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia

    Rashid Mir

  3. Department of Radiotherapy and Oncology, All India Institute of Medical Sciences, New Delhi, India

    P. K. Julka

  4. Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, India

    P. C. Ray & Alpana Saxena

Authors
  1. Jamsheed Javid
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  2. Rashid Mir
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  3. P. K. Julka
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  5. Alpana Saxena
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Correspondence to Alpana Saxena.

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Javid, J., Mir, R., Julka, P.K. et al. Role of survivin re-expression in the development and progression of non-small cell lung cancer. Tumor Biol. 36, 5543–5550 (2015). https://doi.org/10.1007/s13277-015-3224-6

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  • DOI: https://doi.org/10.1007/s13277-015-3224-6

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