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The expression profiles of microRNAs in Kaposi’s sarcoma

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Tumor Biology

Abstract

Kaposi’s sarcoma (KS) is a multicentric angioproliferative tumor of mesenchymal origin. The molecular and biologic aspects of KS are not fully understood. MicroRNAs are non-protein-coding small RNAs in the size range 19–25 nucleotides (nt) that play important roles in biological processes, including cellular differentiation, proliferation, and death. We performed a miRNA microarray analysis by detecting six paired KS and matched adjacent healthy tissues using the 7th generation of miRCURYTM LNA Array (v.18.0) (Exiqon) containing 3100 capture probes. We selected 10 significant differentially expressed miRNAs, which were confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in 18 paired KS and matched adjacent healthy tissue specimens. We also investigated the associations between clinical features and miRNA expression. Among the 3100 human miRNA probes in the microarrays, we identified 170 differentially expressed miRNAs (69 upregulated and 101 downregulated miRNAs) in KS versus adjacent healthy tissues. Among the most significantly upregulated miRNAs were miR-126-3p, miR-199a-3p, miR-16-5p, and the 13 KSHV-related miRNAs. The most significantly downregulated miRNAs included miR-125b-1-3p and miR-1183. Eight upregulated miRNAs, miR-181b-5p, miR-199a-3p, miR-15a-5p, miR-126-3p, miR-1297, kshv-miR-k12-12-3p, kshv-miR-k12-1-5p, and miR-16-5p, and two downregulated miRNAs, miR-125b-1-3p and miR-1183, were confirmed by qRT-PCR in 18 paired KS samples. The qRT-PCR results for 10 miRNAs were consistent with our microarray results. The miR-125b-1-3p and miR-16-5p had statistically significant associations with HHV-8 and HIV infections in KS. The results of miRNA profiling showed that KS appears to have unique expression patterns when compared with paired adjacent healthy tissues, suggesting that deregulation of miRNAs plays an important role in the progression of KS. These differentially expressed miRNAs may provide novel diagnostic and prognostic tools.

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Acknowledgments

This work was supported by a grant from the Natural Science Foundation of China (Grant No. 81260311) and Natural Science Foundation of Xinjiang Uygur Autonomous Region (Grant No. 2014211A059). We are most grateful to all the families who have so willingly participated in this study and made this study possible. We also acknowledge the support of the Clinical Dermatology Institute of Xinjiang Uygur Autonomous Region and National Clinical key specialty construction projects.

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Authors and Affiliations

  1. Department of Dermatology and Venereology, Anhui Medical University, Hefei, Anhui, China

    Xiu-Juan Wu & Xiong-Ming Pu

  2. Department of Dermatology and Venereology, People’s Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China

    Xiu-Juan Wu, Xiong-Ming Pu, Ya-Nan Zhao, Xiao-Jing Kang, Yun-Min Zou, Cao-Ying Wu, Yuan-Yuan Qu, De-Zhi Zhang, Yan-Yan Feng & Jian-Yong Liu

  3. Clinical Medical Research Center, People’s Hospital of Xinjiang, Uygur Autonomous Region, Urumqi, Xinjiang, China

    Zong-Feng Zhao & Wei-Dong Wu

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  1. Xiu-Juan Wu
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Correspondence to Xiong-Ming Pu.

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Xiu-Juan Wu and Xiong-Ming Pu contributed equally to this work and should be considered joint first authors.

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Wu, XJ., Pu, XM., Zhao, ZF. et al. The expression profiles of microRNAs in Kaposi’s sarcoma. Tumor Biol. 36, 437–446 (2015). https://doi.org/10.1007/s13277-014-2626-1

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  • DOI: https://doi.org/10.1007/s13277-014-2626-1

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