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Decreased expression of long noncoding RNA AC096655.1-002 in gastric cancer and its clinical significance

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Tumor Biology

Abstract

Long noncoding RNAs (lncRNAs) are newfound noncoding RNAs that are greater than 200 nucleotides in length. They have emerged recently as major players in governing fundamental biological processes. However, the expression level of lncRNAs and their clinical significances are not well understood. To investigate the lncRNA expression in gastric cancer, real-time reverse transcriptase-polymerase chain reaction was conducted. Then, the association between the level of AC096655.1-002, one of lncRNA, in gastric cancer tissues and the clinicopathological features of patients with gastric cancer was further analyzed. Receiver operating characteristic (ROC) curve was constructed to evaluate the diagnostic values. The results showed that AC096655.1-002 was significantly downregulated in gastric cancer tissues compared with paired adjacent non-tumorous tissues (P < 0.001). Its expression level was significantly correlated with lymph node metastasis (P < 0.001), distant metastasis (P < 0.001), tumor–node–metastasis stages (P < 0.001), and differentiation (P = 0.030). The area under the ROC curve of AC096655.1-002 was up to 0.731. For the detection of gastric cancer, the use of AC096655.1-002 showed a remarkable improvement compared with the use of serum carcinoembryonic antigen. These results indicated that lncRNA AC096655.1-002 may be a potential biomarker in the diagnosis of gastric carcinoma.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (no. 81171660), Natural Science Foundation of Ningbo (no. 2012A610207), the Scientific Innovation Team Project of Ningbo (no. 2011B82014), the Project of Key Disciplines in Ningbo (no. XKL11D2127 and XKL11D2128), the Research and Development Projects of Experimental Techniques in Ningbo University (no. SYJS-201103), College Students’ Science-Technology Innovation Program of Zhejiang Province (no. 200959 and 2011R405033), the Excellent Dissertation Fund of Ningbo University (no. PY2012004), and the K. C. Wong Magna Fund in Ningbo University.

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Authors and Affiliations

  1. Ningbo Yinzhou People’s Hospital and the Affiliated Hospital, Ningbo University School of Medicine, Ningbo, 315040, China

    Weiliang Sun

  2. Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China

    Yibo Wu, Xing Yu, Yang Liu, Haojun Song, Tian Xia, Bingxiu Xiao & Junming Guo

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  1. Weiliang Sun
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  2. Yibo Wu
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  3. Xing Yu
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  4. Yang Liu
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  6. Tian Xia
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  7. Bingxiu Xiao
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Correspondence to Junming Guo.

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Sun, W., Wu, Y., Yu, X. et al. Decreased expression of long noncoding RNA AC096655.1-002 in gastric cancer and its clinical significance. Tumor Biol. 34, 2697–2701 (2013). https://doi.org/10.1007/s13277-013-0821-0

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  • DOI: https://doi.org/10.1007/s13277-013-0821-0

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