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An integrated mRNA–microRNA regulatory network identified INHBA and has-miR-135a-5p as predictors of gastric cancer recurrence

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Abstract

Backgrounds

Gastric cancer (GC), a prevalent malignancy in Eastern Asia, is associated with aberrant transcriptional regulation.

Objective

Here, we evaluated the mRNA and microRNA transcriptomes in patients with gastric cancer to gain insight into the molecular underpinnings of this disease.

Results

We observed upregulation of inhibin βA (INHBA), CDC7, SULF1, COL11A1, KIAA1199, and CLDN1 transcripts in gastric cancer and showed that INHBA upregulation was associated with cancer recurrence. Expression of has-miR-135a-5p was significantly lower in gastric cancer tissues compared with matched normal tissues and was inversely associated with INHBA expression.

Conclusion

Our findings suggest that INHBA and has-miR-135a-5p expression serve as therapeutic markers of gastric cancer recurrence.

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Acknowledgements

This research was supported by the National R&D Program for Cancer Control, Ministry of Health and Welfare (HA17C0054) and the Hallym University Research Fund. The funding agencies were not involved in the study design, data collection or analysis, decision to publish, or preparation of the manuscript.

Author information

Author notes

  1. Sung-Hwa Sohn, Hee Jung Sul, Yeonsong Choi and Semin Lee contributed equally to this work.

Authors and Affiliations

  1. Hallym Translational Research Institute, Hallym University Sacred Heart Hospital, Anyang, 14066, Republic of Korea

    Sung-Hwa Sohn, Hee Jung Sul & Dae Young Zang

  2. Department of Internal Medicine, Hallym University Medical Center, Hallym University College of Medicine, Anyang, Gyeonggi-do, 14068, Republic of Korea

    Bum Jun Kim, Hyeong Su Kim & Dae Young Zang

  3. Department of Bio-Medical Gerontology, Ilsong Institute of Life Sciences, Hallym University, Anyang, Gyeonggi-do, Republic of Korea

    Youngho Koh

  4. Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea

    Yeonsong Choi & Semin Lee

Authors
  1. Sung-Hwa Sohn
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  2. Hee Jung Sul
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  3. Yeonsong Choi
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  4. Semin Lee
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  5. Bum Jun Kim
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  8. Dae Young Zang
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Contributions

SHS performed molecular experiments, analyzed data, and drafted the article. HJS performed the experiments. YC and SL analyzed the miRNA and mRNA data. BJK, HS K, and YK critically revised the manuscript for important intellectual content. DYZ supervised the study, obtained funding, guided the analyses of data, and edited the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Dae Young Zang.

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Conflict of interest

Sung-Hwa Sohn, Hee Jung Sul, Yeonsong Choi, Semin Lee, Bum Jun Kim, Hyeong Su Kim, Youngho Koh and Dae Young Zang declare that they have no conflict of interest.

Human and animal rights

All human RNA samples were approved by the Institutional Review Board of the Hallym University.

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Sohn, SH., Sul, H.J., Choi, Y. et al. An integrated mRNA–microRNA regulatory network identified INHBA and has-miR-135a-5p as predictors of gastric cancer recurrence. Mol. Cell. Toxicol. 17, 213–220 (2021). https://doi.org/10.1007/s13273-021-00127-8

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  • DOI: https://doi.org/10.1007/s13273-021-00127-8

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