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Identification of lung cancer specific differentially methylated regions using genome-wide DNA methylation study

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Abstract

Backgrounds

Many molecular biomarkers have been suggested for the diagnosis, prognosis, and treatment response of lung cancer, but their clinical availability remains limited. DNA methylation is one such promising biomarker because it is stable and easily detected.

Methods

We conducted an epigenome-wide analysis using methyl binding domain (MBD) sequencing in tissues of lung cancer patients. Tumor and normal tissues were obtained from two patients who underwent surgery for non-small cell lung cancer.

Results

HOXA5, HOXA9, and other related genes were associated with the CGI-located DMRs in Patient 1, whereas the SSTR5 gene was found to be associated with the CGI-located DMRs in Patient 2. Interestingly, these DMR genes were mapped in the physical interaction networks that included previously known nonsmall cell lung cancer genes.

Conclusion

This genome-wide DNA methylation study showed an association between newly identified DMRs in CpG island promoter regions and previously known target genes for lung cancer.

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Authors and Affiliations

  1. Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea

    Yoonki Hong, Seok-Ho Hong & Woo Jin Kim

  2. Department of Pulmonary and Critical Care Medicine, and Clinical Research Center for Chronic Obstructive Airway Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea

    Yeon-Mok Oh

  3. Division of Biomedical Convergence, College of Biomedical Science, and Institute of Bioscience & Biotechnology, Kangwon National University, Chuncheon, 24341, Republic of Korea

    Seung-Ho Shin & Sun Shim Choi

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  1. Yoonki Hong
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  2. Seok-Ho Hong
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  4. Seung-Ho Shin
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Correspondence to Sun Shim Choi or Woo Jin Kim.

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Hong, Y., Hong, SH., Oh, YM. et al. Identification of lung cancer specific differentially methylated regions using genome-wide DNA methylation study. Mol. Cell. Toxicol. 14, 315–322 (2018). https://doi.org/10.1007/s13273-018-0034-0

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  • DOI: https://doi.org/10.1007/s13273-018-0034-0

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