Skip to main content
SpringerLink
Log in

Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells

  • Original Paper
  • Published:
Molecular & Cellular Toxicology Aims and scope Submit manuscript

Abstract

Vascular inflammation is an important hallmark of high-fat-induced atherosclerosis. Oxidized low-density lipoprotein (ox-LDL) is a key initiator of inflammation as it induces inflammatory gene expression in vascular endothelial cells. Sodium ferulate (SF), an active component from Chinese medicine, demonstrated anti-atherosclerotic potency. However, the mechanism is unknown. Here we investigated how SF changed the cellular gene expression profile and restored ox-LDL-triggered inflammation in HUVECs. Gene expression profile, inflammatory gene expression and NF-κB activation were investigated in human umbilical vein endothelial cells (HUVECs) with or without SF (5 μM) treatment after precondition with ox-LDL (50 μg/mL). Ox-LDL treatment increased the production of IL-1β, CCL20, IL-6, IL-8 and CXCL1. SF stimulation modulated the translocation of NF-κB between cytoplasm and nucleus, and alleviated the inflammatory response induced by ox-LDL. Collectively, SF could suppress the expression of inflammatory factors in ox-LDL-stimulated endothelial cells, and transcription factor NF-κB might be involved in such process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ermak, N., Lacour, B., Drueke, T. B. & Vicca, S. Role of reactive oxygen species and Bax in oxidized low density lipoprotein-induced apoptosis of human monocytes. Atherosclerosis 200:247–256 (2008).

    Article  CAS  PubMed  Google Scholar 

  2. Ross, R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362:801–809 (1993).

    Article  CAS  PubMed  Google Scholar 

  3. Luscinskas, F. W. & Gimbrone, M. A. Endothelialdependent mechanisms in chronic inflammatory leukocyte recruitment. Annu Rev Med 47:413–421 (1996).

    Article  CAS  PubMed  Google Scholar 

  4. Mantovani, A., Bussolino, F. & Introna, M. Cytokine regulation of endothelial cell function: from molecular level to the bedside. Immunol Today 18:231–240 (1997).

    Article  CAS  PubMed  Google Scholar 

  5. Pahl, H. L. Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene 18:6853–6866 (1999).

    Article  CAS  PubMed  Google Scholar 

  6. Thurberg, B. L. & Collins, T. The nuclear factor-kappa B/inhibitor of kappa B autoregulatory system and atherosclerosis. Curr Opin Lipidol 9:387–396 (1998).

    Article  CAS  PubMed  Google Scholar 

  7. Barnes, P. J. & Karin, M. Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med 336:1066–1071 (1997).

    Article  CAS  PubMed  Google Scholar 

  8. Makarov, S. S. NF-kappaB as a therapeutic target in chronic inflammation: recent advances. Mol Med Today 6:441–448 (2000).

    Article  CAS  PubMed  Google Scholar 

  9. Tak, P. P. & Firestein, G. S. NF-kappaB: a key role in inflammatory diseases. J Clin Invest 107:7–11 (2001).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Ju, H. S. et al. Scavenging effects of sodium ferulate and 18 beta-glycyrrhetic acid on oxygen free radicals. Zhongguo Yao Li Xue Bao 11:466–470 (1990).

    CAS  PubMed  Google Scholar 

  11. Mathew, S. & Abraham, T. E. Ferulic acid: an antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications. Crit Rev Biotechnol 24:59–83 (2004).

    Article  CAS  PubMed  Google Scholar 

  12. Ogiwara, T. et al. Radical scavenging activity and cytotoxicity of ferulic acid. Anticancer Res 22:2711–2717 (2002).

    CAS  PubMed  Google Scholar 

  13. Wang, B. H. & Ou-Yang, J. P. Pharmacological actions of sodium ferulate in cardiovascular system. Cardiovasc Drug Rev 23:161–172 (2005).

    Article  PubMed  Google Scholar 

  14. Wang, B. et al. Sodium ferulate inhibits atherosclerogenesis in hyperlipidemia rabbits. J Cardiovasc Pharmacol 43:549–554 (2004).

    Article  CAS  PubMed  Google Scholar 

  15. Cominacini, L. et al. Oxidized low density lipoprotein (ox-LDL) binding to ox-LDL receptor-1 in endothelial cells induces the activation of NF-kappaB through an increased production of intracellular reactive oxygen species. J Biol Chem 275:12633–12638 (2000).

    Article  CAS  PubMed  Google Scholar 

  16. Xiaohong, Y., Jing-Ping, O. Y. & Shuzheng, T. Angelica protects the human vascular endothelial cell from the effects of oxidized low-density lipoprotein in vitro. Clin Hemorheol Microcirc 22:317–323 (2000).

    CAS  PubMed  Google Scholar 

  17. Havel, R. J., Eder, H. A. & Bragdon, J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 34:1345–1353 (1955).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Cominacini, L. et al. A simple test for predisposition to LDL oxidation based on the fluorescence development during copper-catalyzed oxidative modification. J Lipid Res 32:349–358 (1991).

    CAS  PubMed  Google Scholar 

  19. Littlewood, T. D. & Bennett, M. R. Apoptotic cell death in atherosclerosis. Curr Opin Lipidol 14:469–475 (2003).

    Article  CAS  PubMed  Google Scholar 

  20. Zhang, R., Ma, J., Xia, M., Zhu, H. & Ling, W. Mild hyperhomocysteinemia induced by feeding rats diets rich in methionine or deficient in folate promotes early atherosclerotic inflammatory processes. J Nutr 134:825–830 (2004).

    CAS  PubMed  Google Scholar 

  21. Galle, J., Hansen-Hagge, T., Wanner, C. & Seibold, S. Impact of oxidized low density lipoprotein on vascular cells. Atherosclerosis 185:219–226 (2006).

    Article  CAS  PubMed  Google Scholar 

  22. Sun, J. et al. Mast cells promote atherosclerosis by releasing proinflammatory cytokines. Nat Med 13:719–724 (2007).

    Article  CAS  PubMed  Google Scholar 

  23. Tedgui, A. & Mallat, Z. Cytokines in atherosclerosis: pathogenic and regulatory pathways. Physiol Rev 86:515–581 (2006).

    Article  CAS  PubMed  Google Scholar 

  24. Murphy, P. M. et al. International union of pharmacology. XXII. Nomenclature for chemokine receptors. Pharmacol Rev 52:145–176 (2000).

    CAS  PubMed  Google Scholar 

  25. Zlotnik, A. & Yoshie, O. Chemokines: a new classification system and their role in immunity. Immunity 12:121–127 (2000).

    Article  CAS  PubMed  Google Scholar 

  26. Kuziel, W. A. et al. Severe reduction in leukocyte adhesion and monocyte extravasation in mice deficient in CC chemokine receptor 2. Proc Natl Acad Sci USA 94:12053–12058 (1997).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Baeuerle, P. A. & Baltimore, D. NF-kappa B: ten years after. Cell 87:13–20 (1996).

    Article  CAS  PubMed  Google Scholar 

  28. Dandona, P., Chaudhuri, A., Ghanim, H. & Mohanty, P. Proinflammatory effects of glucose and anti-inflammatory effect of insulin: relevance to cardiovascular disease. Am J Cardiol 99:15B–26B (2007).

    Article  CAS  PubMed  Google Scholar 

  29. Harokopakis, E., Albzreh, M. H., Haase, E. M., Scannapieco, F. A. & Hajishengallis, G. Inhibition of proinflammatory activities of major periodontal pathogens by aqueous extracts from elder flower (Sambucus nigra). J Periodontol 77:271–279 (2006).

    Article  PubMed  Google Scholar 

  30. Kwon, K. B. et al. Sophorae radix extract inhibits high glucose-induced vascular cell adhesion molecule-1 upregulation on endothelial cell line. Clin Chim Acta 348:79–86 (2004).

    Article  CAS  PubMed  Google Scholar 

  31. Lee, Y. J., Kang, D. G., Kim, J. S. & Lee, H. S. Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells. Vascul Pharmacol 48:38–46 (2008).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of public health, Wuhan University, Wuhan, 430072, China

    Junliang Tao, Yonghong Man, Weina Wang & Yongyi Bi

  2. Nanyang Medical College, Nanyang, 473000, China

    Junliang Tao, Dongxian Zhang, Yonghong Man & Weina Wang

Authors
  1. Junliang Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dongxian Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yonghong Man
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Weina Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yongyi Bi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yongyi Bi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tao, J., Zhang, D., Man, Y. et al. Sodium ferulate inhibits high-fat diet-induced inflammatory factors expression in human umbilical vein endothelial cells. Mol. Cell. Toxicol. 12, 117–123 (2016). https://doi.org/10.1007/s13273-016-0015-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13273-016-0015-0

Keywords

Search

Navigation