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Evaluation of substance-P toxicity with single dose and repeated dose in rats

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Abstract

Toxicity study evaluating the effects of intravenous injection of SP with single- and repeated-dose in rats was performed. Single 100, 50, and 10 mg/kg doses of SP were administered and animals were monitored for 14 days. Although 10 mg/kg dose did not affect behavior and survival, 100 and 50 mg/kg doses elicited mortality, suggesting the minimum lethal dose (MLD) of SP to be between 10 and 50 mg/kg. To evaluate the toxicity of repeated dosing, rats were injected twice first two days per week with 30, 10, and 3 mg/kg of SP over 2 weeks and evaluated for survival, behavior, hematological factors, and organ weight. All rats administered with 10 and 3 mg/kg SP survived with minor changes in behavior and hematological indices. Thus, certain poison dose was estimated to be approximately 3 mg/kg, indicating that application of SP below 3mg/kg may not be associated with toxicity.

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Authors and Affiliations

  1. College of Medicine/ East-West Medical Research Institute, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-702, Korea

    Hyun Sook Hong

  2. Department of Clinical Pharmacology, School of Medicine, Kyung Hee University, 1 Hoegi-dong Dongdaemun-gu, Seoul, 130-702, Korea

    Sung Vin Lim

  3. Department of Genetic Engineering, College of Life Science and Graduate School of Biotechnology, Kyung Hee University, Seochun-dong, Kiheung-gu, Yong In, 441-706, Korea

    Youngsook Son

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  1. Hyun Sook Hong
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Correspondence to Youngsook Son.

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Hong, H.S., Lim, S.V. & Son, Y. Evaluation of substance-P toxicity with single dose and repeated dose in rats. Mol. Cell. Toxicol. 11, 201–211 (2015). https://doi.org/10.1007/s13273-015-0019-1

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  • DOI: https://doi.org/10.1007/s13273-015-0019-1

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