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Immobilization of para-nitrobenzyl esterase-CLEA on electrospun polymer nanofibers for potential use in the synthesis of cephalosporin-derived antibiotics

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Abstract

The para-nitrobenzyl esterase (PNBE) was successfully immobilized onto electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The maximum amount of PNBE immobilized on the PSPSMA nanofibers was determined to be 56.4 mg/g of nanofibers. The variation of pH and temperature has been observed to affect the enzymatic activity of the free and immobilized PNBE-CLEA. The immobilized PNBE-CLEA has been observed to show improved stability as compared to the native one. PNBE-CLEA has been found to retain more than 63% activity after 60 consecutive reuses. Finally, the immobilized PNBECLEA presented a high decomposition efficiency of cephalexin tosylate PNB ester. The durability determination of PNBE-CLEA immobilized nanofibers showed 70% of de-esterification after 10 cycles. These results demonstrate that PNBE-CLEA immobilized nanofiber could be used as an economical and environment friendly system for the catalytic process of paranitrobenzyl esters in pharmaceutical synthesis.

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Authors and Affiliations

  1. Department of Microbiology, Chungbuk National University, 52 Naesudong-Ro, Heungduk-Gu, Cheongju, 361-763, Korea

    Simranjeet Singh Sekhon, Jae-Min Park, Ji-Young Ahn, Yoon-Chul Kim & Yang-Hoon Kim

  2. Division of Endocrinology & Metabolism, Department of Internal Medicine, Chonbuk National University Medical School, 634-18 Geumam-Dong, Duckjin-Gu, Jeonju, 561-712, Korea

    Tae Sun Park

  3. Institute of Mine Reclamation Technology, 362 Maeju-Ri, Seonghwan-Eup, Seobuk-Gu, Cheonan, 331-803, Korea

    Soon-Dong Kwon

  4. Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14, 1-Ga, Duckjin-Dong, Duckjin-Gu, Jeonju, 561-156, Korea

    Jiho Min

Authors
  1. Simranjeet Singh Sekhon
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  2. Jae-Min Park
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  3. Ji-Young Ahn
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  4. Tae Sun Park
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  5. Soon-Dong Kwon
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  6. Yoon-Chul Kim
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  7. Jiho Min
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  8. Yang-Hoon Kim
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Corresponding authors

Correspondence to Jiho Min or Yang-Hoon Kim.

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These authors contributed equally to this work.

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Sekhon, S.S., Park, JM., Ahn, JY. et al. Immobilization of para-nitrobenzyl esterase-CLEA on electrospun polymer nanofibers for potential use in the synthesis of cephalosporin-derived antibiotics. Mol. Cell. Toxicol. 10, 215–221 (2014). https://doi.org/10.1007/s13273-014-0023-x

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  • DOI: https://doi.org/10.1007/s13273-014-0023-x

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