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Physiological and omics analysis of maize inbred lines during late grain development

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Abstract

Background

There were significant differences in the change of moisture content and grain composition at the late stage of grain development among different maize varieties, but the regulation mechanism is not clear.

Objective

To explore the key genes causing the variation in physiological traits of two typical maize inbred lines in late grain development.

Methods

The grains at different development stages were selected as materials to determine the content of water, sucrose, starch and ABA. Transcriptomic and proteomic analysis of the materials were performed to screen relevant genes.

Results

The grain dehydration rate and the content of sucrose, starch and ABA were showed significant differences between two varieties in the late stage of grain development. The enrichment analysis of common differentially expressed genes (proteins) showed that most of the genes (proteins) were enriched in the extracellular region. The downregulated genes were mainly concentrated in carbohydrate metabolism and lipid metabolism, while the upregulated genes were mainly in response to stress. Furthermore, this study also identified many key candidate genes (dehydrin genes, pathogenesis-related genes, sucrose synthase and secondary metabolites related genes) related to late grain development of maize.

Conclusions

The suggested genes related to late grain development of maize can be candidates for further functional study.

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Acknowledgements

The authors are grateful for the transcriptome and iTRAQ analyses performed by the BGI Gene Co., Ltd. (Shenzhen, China).

Funding

This study was funded by Key Science and Technology Projects in Henan Province (Grant no. 212102110305) and Important Science and Technology Specific Projects in Henan Province (Grant no. 201300111100).

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Authors and Affiliations

  1. Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang, 453003, China

    Xining Jin, Huijie Zhai, Pingxi Wang, Xiaoxiang Zhang, Xiangyuan Wu, Huaisheng Zhang, Shilin Chen & Zhongwen Huang

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  1. Xining Jin
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  2. Huijie Zhai
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  4. Xiaoxiang Zhang
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  8. Zhongwen Huang
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Contributions

SLC, XNJ, and ZWH conceived and designed research. XNJ, PXW and XXZ conducted the experiments. XNJ and XYW contributed new reagents or analytical tools. HJZ and XNJ analyzed the data. XNJ wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Xining Jin.

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Jin, X., Zhai, H., Wang, P. et al. Physiological and omics analysis of maize inbred lines during late grain development. Genes Genom 44, 993–1006 (2022). https://doi.org/10.1007/s13258-022-01279-0

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