Skip to main content
SpringerLink
Log in

LncSNHG3 promotes hepatocellular carcinoma epithelial mesenchymal transition progression through the miR-152-3p/JAK1 pathway

  • Research Article
  • Published:
Genes & Genomics Aims and scope Submit manuscript

Abstract

Background

The dysregulation of LncRNAs is related to the malignant progression of many cancers.

Objective

The study aimed to investigate the expression and the biological role of LncSNHG3 in hepatocellular carcinoma (HCC).

Methods

The TCGA data of the LncSNHG3 in HCC were analyzed. The expression in HCC cell lines was detected by qRT-PCR. Proliferation, migration, and invasion of HepG2 and Huh7 were examined by cell counting kit-8, colony formation, transwell assays, and wound healing assays. At the same time, the interactions among LncSNHG3, miR-152-3p, and JAK1 were confirmed by dual-luciferase reporter assay, RNA immunoprecipitation, subcellular distribution. Xenograft tumor-bearing mice models were used to measure the effect of LncSNHG3 on the growth of HCC in vivo. The apoptosis and epithelial mesenchymal transition (EMT)-associated proteins were checked by WB and IHC.

Results

LncSNHG3 was overexpressed in HCC tissues and cell lines. In addition, it is correlated with the tumor stage and survival time of HCC patients. Down-regulated LncSNHG3 could significantly suppress the EMT progression of HCC in vivo and in vitro. LncSNHG3 could promote the JAK1 expression by sponging miR-152-3p.

Conclusions

LncSNHG3 acted as an oncogene and promoted the EMT procession in HCC by binding miR-152-3p and promoting JAK1 expression. Predictably, LncSNHG3 was used as a potential marker and will be used as a novel therapy target for HCC in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Availability of data and material

The data are available from the corresponding author on reasonable request.

References

  • Bhan A, Soleimani M, Mandal SS (2017) Long noncoding RNA and cancer: a new paradigm. Cancer Res 77:3965–3981

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cao Y, Zhang F, Wang H, Bi C, Cui J, Liu F, Pan H (2021) LncRNA MALAT1 mediates doxorubicin resistance of hepatocellular carcinoma by regulating miR-3129-5p/Nova1 axis. Mol Cell Biochem 476:279–292

    Article  CAS  PubMed  Google Scholar 

  • Ding D, Li C, Zhao T, Li D, Yang L, Zhang B (2018) LncRNA H19/miR-29b-3p/PGRN axis promoted epithelial-mesenchymal transition of colorectal cancer cells by acting on wnt signaling. Mol Cells 41:423–435

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136:E359–E386

    Article  CAS  PubMed  Google Scholar 

  • Forner A, Reig M, Bruix J (2018) Hepatocellular carcinoma. Lancet 391:1301–1314

    Article  PubMed  Google Scholar 

  • Guttman M, Rinn JL (2012) Modular regulatory principles of large non-coding RNAs. Nature 482:339–346

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jarroux J, Morillon A, Pinskaya M (2017) History, discovery, and classification of lncRNAs. Adv Exp Med Biol 1008:1–46

    Article  CAS  PubMed  Google Scholar 

  • Llovet JM, Montal R, Sia D, Finn RS (2018) Molecular therapies and precision medicine for hepatocellular carcinoma. Nat Rev Clin Oncol 15:599–616

    Article  PubMed  Google Scholar 

  • Lou J, Yan W, Li QY, Zhu AK, Tan BQ, Dong R, Zou XZ, Liu T (2021) LncRNA MEG8 plays an oncogenic role in hepatocellular carcinoma progression through miR-367-3p/14-3-3zeta/TGFbetaR1 axis. Neoplasma 68:273–282

    Article  PubMed  Google Scholar 

  • Morse MA, Sun W, Kim R, He AR, Abada PB, Mynderse M, Finn RS (2019) The role of angiogenesis in hepatocellular carcinoma. Clin Cancer Res 25:912–920

    Article  CAS  PubMed  Google Scholar 

  • Santucci R, Sinibaldi F, Cozza P, Polticelli F, Fiorucci L (2019) Cytochrome c: An extreme multifunctional protein with a key role in cell fate. Int J Biol Macromol 136:1237–1246

    Article  CAS  PubMed  Google Scholar 

  • Statello L, Guo CJ, Chen LL, Huarte M (2021) Gene regulation by long non-coding RNAs and its biological functions. Nat Rev Mol Cell Biol 22:96–118

    Article  CAS  PubMed  Google Scholar 

  • Subramaniam A, Shanmugam MK, Ong TH, Li F, Perumal E, Chen L, Vali S, Abbasi T, Kapoor S, Ahn KS et al (2013) Emodin inhibits growth and induces apoptosis in an orthotopic hepatocellular carcinoma model by blocking activation of STAT3. Br J Pharmacol 170:807–821

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tao H, Li J, Liu J, Yuan T, Zhang E, Liang H, Huang Z (2021) Construction of a ceRNA network and a prognostic lncRNA signature associated with vascular invasion in hepatocellular carcinoma based on weighted gene co-expression network analysis. J Cancer 12:3754–3768

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wan T, Zheng J, Yao R, Yang S, Zheng W, Zhou P (2021) LncRNA DDX11-AS1 accelerates hepatocellular carcinoma progression via the miR-195-5p/MACC1 pathway. Ann Hepatol 20:100258

    Article  CAS  PubMed  Google Scholar 

  • Weng W, Zhang Z, Huang W, Xu X, Wu B, Ye T, Shan Y, Shi K, Lin Z (2020) Identification of a competing endogenous RNA network associated with prognosis of pancreatic adenocarcinoma. Cancer Cell Int 20:231

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu J, Liu L, Jin H, Li Q, Wang S, Peng B (2019) LncSNHG3/miR-139-5p/BMI1 axis regulates proliferation, migration, and invasion in hepatocellular carcinoma. Onco Targets Ther 12:6623–6638

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yin T, Liu MM, Jin RT, Kong J, Wang SH, Sun WB (2019) miR-152-3p Modulates hepatic carcinogenesis by targeting cyclin-dependent kinase 8. Pathol Res Pract 215:152406

    Article  CAS  PubMed  Google Scholar 

  • Zhang T, Cao C, Wu D, Liu L (2016) SNHG3 correlates with malignant status and poor prognosis in hepatocellular carcinoma. Tumour Biol 37:2379–2385

    Article  CAS  PubMed  Google Scholar 

  • Zhang PF, Wang F, Wu J, Wu Y, Huang W, Liu D, Huang XY, Zhang XM, Ke AW (2019) LncRNA SNHG3 induces EMT and sorafenib resistance by modulating the miR-128/CD151 pathway in hepatocellular carcinoma. J Cell Physiol 234:2788–2794

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Kodali S, Chen M, Wang J (2020) Maintenance of germinal center B cells by caspase-9 through promotion of apoptosis and inhibition of necroptosis. J Immunol 205:113–120

    Article  CAS  PubMed  Google Scholar 

  • Zhu GJ, Song PP, Zhou H, Shen XH, Wang JG, Ma XF, Gu YJ, Liu DD, Feng AN, Qian XY et al (2018) Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, beta-catenin and ZEB2 in laryngeal squamous cell carcinoma. Oncol Lett 15:3472–3481

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The study was supported by Natural Science Foundation of China (81860534); Inner Mongolia autonomous region science and technology planning project (2019GG039/086/107, 2021GG0167); Zhi Yuan Talent Projects of Inner Mongolia Medical University (ZY0202011); Natural Science Foundation of Inner Mongolia (2021MS08152).

Author information

Author notes

  1. Hong Li, Yu Wu and Runmei Wang have contributed equally to this work and share first authorship

Authors and Affiliations

  1. Department of Radiation Oncology, Inner Mongolia Cancer Hospital and Affiliated People’s Hospital of Inner Mongolia Medical University, 010020, Huhhot, China

    Hong Li, Runmei Wang, Junmei Guo, Qin Yu, Lihe Zhang & Hao Yang

  2. Department of Nuclear Medicine, Inner Mongolia Cancer Hospital and Affiliated People’s Hospital of Inner Mongolia Medical University, 010020, Huhhot, China

    Yu Wu

  3. Department of Abdominal Tumor Surgery, Affiliated Hospital of Inner Mongolia Medical University, 010020, Huhhot, China

    Haiping Zhao

  4. The Laboratory of radiation physics and biology, Inner Mongolia Cancer Hospital and Affiliated People’s Hospital of Inner Mongolia Medical University, 010020, Huhhot, China

    Hong Li, Runmei Wang, Junmei Guo, Qin Yu, Lihe Zhang & Hao Yang

Authors
  1. Hong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Runmei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Junmei Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Qin Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lihe Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Haiping Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hao Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Haiping Zhao and Hao Yang designed the study. Hong Li, Yu Wu and Runmei Wang performed the experiments and drafted the manuscript. Junmei Guo, Qin Yu and Lihe Zhang analyzed the data. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Haiping Zhao or Hao Yang.

Ethics declarations

Conflict of interest

Hong Li, Yu Wu, Runmei Wang, Junmei Guo, Qin Yu, Lihe Zhang, Haiping Zhao and Hao Yang declare that there are no conflicts of interest.

Ethics approval

The animal experiments were approved by the Inner Mongolia Medical University Animal Ethics Committee and performed according to the Guide for the Care and Use of Laboratory Animals.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, H., Wu, Y., Wang, R. et al. LncSNHG3 promotes hepatocellular carcinoma epithelial mesenchymal transition progression through the miR-152-3p/JAK1 pathway. Genes Genom 44, 133–144 (2022). https://doi.org/10.1007/s13258-021-01185-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13258-021-01185-x

Keywords

Search

Navigation