Abstract
Pollen development is disturbed in the microspore development stage of the double-recessive nuclear male-sterile line ms5ms6 (Gossypium hirsutum L.). This study aimed to identify differentially expressed anther proteins and their potential roles in pollen development and male sterility. We compared the proteomes of sterile and fertile anthers of the double recessive nuclear male-sterile line ms5ms6. Approximately 1,390 protein spots were detected by two-dimensional differential gel electrophoresis. Proteins with altered accumulation levels in sterile anthers compared with fertile anthers were identified by mass spectrometry and the NCBInr and Viridiplantae EST databases. Down-regulated proteins in the sterile anthers included cytosolic ascorbate peroxidase 1 and glutaminyl-tRNA synthetase (glutamine-tRNA ligase). Several carbohydrate metabolism- and photosynthesis-related enzymes were also present at lower levels in the mutant anthers. By contrast, ATP-dependent RNA helicase eIF4A-13, NADH dehydrogenase subunit 1, enolase, gibberellin 20-oxidase, gibberellin 3-hydroxylase 1, alcohol dehydrogenase 2d, 3-ketoacyl-CoA synthase, and trehalose 6-phosphate synthase were expressed at higher levels in sterile anthers than in fertile anthers. The regulation of upland cotton pollen development involves a complex network of differentially expressed genes. This study provides the foundation for future investigations of gene function in upland cotton pollen development and male sterility.
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Abbreviations
- GMS:
-
Genic male sterility
- GS:
-
Genic male sterility
- 2-DE:
-
Two-dimensional electrophoresis
- IPG:
-
pH gradient
- MALDI-TOF–TOF:
-
Matrix-assisted laser desorption/ionization time-of-flight and time-of-flight
- NCBI:
-
National Center for Biotechnology Information
- PCD:
-
Programmed cell death
- CMS:
-
Cytoplasmic male sterility
- ORF:
-
Open reading frames
- TCA:
-
Trichloroacetic acid
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Acknowledgments
We thank the other members of our laboratory for help in the research and for insightful remarks. This work was supported by the Natural Science Foundation of Tianjin (12JCQNJC09700, 12JCZDJC23000), the Science and Technology Development Foundation Program of the University in Tianjin (20100606), Doctoral Fund of Tianjin Normal University (52XB1105), Henry Fok Education Fund (20111203210002), and Open Fund of Tianjin Key Laboratory of Animal and Plant Resistance (52XS1210). Thank the referees for helpful comments.
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The authors declared that they have no conflicts of interest to this work.
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Yan Ren is the co-first author.
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Yue, J., Ren, Y., Wu, S. et al. Differential proteomic studies of the genic male-sterile line and fertile line anthers of upland cotton (Gossypium hirsutum L.). Genes Genom 36, 415–426 (2014). https://doi.org/10.1007/s13258-014-0176-y
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DOI: https://doi.org/10.1007/s13258-014-0176-y