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Fine mapping and candidate gene analysis of the dwarf gene d162(t) in rice (Oryza sativa L.)

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Abstract

In our previous study, d162(t), a single recessive gene, which caused rice dwarf mutant, had been mapped on the short arm of chromosome 3. In this study, the d162(t) gene was fine mapped to a confined region about 0.82 cM by RM14641 and RM3134, and co-segregated with InDel361-2, InDel361-3, InDel361-5, RM14645, RM1022 and RM14643, where no known gene involved in plant height has been identified. Based on the annotation results of TIGR, dozens of open reading frames (ORFs) were predicted in this region, among them, five ORFs were the most possible genes related to the phenotype. In these ORFs, Os03g13010, related to U-box domain containing protein, had a 62bp segment deletion in the coding region in 162d (mutant type, MT). The results of RT-PCR showed that the transcriptional level of Os03g13010 was significantly different between Shuhui162 (wild type, WT) and 162d (MT). Therefore, the gene (Os03g13010) encoding a U-box domain containing protein was considered as the candidate gene of d162(t).

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Authors and Affiliations

  1. Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, P. R. China

    Fan-tao Zhang, Ping-rong Wang, Chang-hui Sun, Bing Wang, Xiu-lan Li, Jian-qing Zhu, Xiao-ling Gao & Xiao-jian Deng

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  1. Fan-tao Zhang
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  2. Ping-rong Wang
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  3. Chang-hui Sun
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  4. Bing Wang
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Correspondence to Xiao-jian Deng.

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Zhang, Ft., Wang, Pr., Sun, Ch. et al. Fine mapping and candidate gene analysis of the dwarf gene d162(t) in rice (Oryza sativa L.). Genes Genom 33, 25–30 (2011). https://doi.org/10.1007/s13258-010-0056-z

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