Abstract
Sesame is a well-known and primordial oilseed crop. The commonly cultivated Indian sesame (S. indicum) seed accumulates more than 50% oil and is pale yellow in colour at maturity. On the contrary, wild S. mulayanum is a low oil-containing (< 50%) genotype, with brownish black seed coat colour. The genic foundation of sesame oil quantity, quality and seed coat colour remains poorly known due to its intricacy. The present study examines the transcriptome of developing seeds from two sesame types, S. indicum and S. mulayanum, and sheds insight on the genes involved in oil biosynthesis and seed coat colour. We have carried out RNA sequencing of developing seeds at 10 and 30 DAP (days after pollination) from two genotypes and performed differential expression study. The high oil containing cultivated sesame revealed high expression of the key lipid biosynthesis genes like acetyl-CoA carboxylase, glycerol-3-phosphate acyltransferase, choline phospho transferase, GDSL esterase or lipase, lipid transfer protein, and carboxylesterase. Furthermore, many transcription factors were differentially expressed during seed maturation, including bHLH30, PIF1, ASIL2, and WRKY. The genes controlling seed coat colour included polyphenol oxidases, NAC domain-containing protein 43, and pentatricopeptide repeat-containing proteins. Several transcription factors controlling anthocyanin biosynthesis, such as GATA 18-like90, zinc finger protein, WRKY, PIF1, and ASIL2 showed significant alterations in their expression levels. This study generated a considerable transcriptome dataset and gene list controlling oil production and seed coat colour modulation in sesame, which we envisage to validate through functional studies.
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Data availability
The raw illumina sequences were deposited as FASTQ format in the NCBI Sequence Read Archive (SRA) with accession identity PRJNA644139 (accession numbers SRS6952386 and SRS6952390). The R-Script (Gene Info sorter_V4.R) used for transcriptomics data processing is available on GitHub (https://github.com/debabratadutta6/Sesame-transcriptome/blob/main/Gene%20Info%20sorter_V4.R) and is licensed under GPLv3.
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Acknowledgements
DD acknowledges Dr Amit P Parikh, Department of Biotechnology (DBT), India and Purvaja Ramachandran, National Biodiversity Authority, India for providing necessary permission for carrying sesame samples to the University of York, UK. GG is indebted to the Director, Bose Institute for providing the infrastructural support and an intramural research grant. DD and GG are thankful to Mr Jadab Ghosh, Mrs Kaberi Ghosh and Mrs Sheolee Chakraborty for their technical assistance.
Funding
This work was funded by a grant (Grant Number: BT/IN/NBPP/DD/04/2018-19) under theNewton Bhabha PhD Placement Programme, jointly supported by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India and the British Council, UK.
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DD: Conceptualization, Methodology, Formal analysis, Investigation, Data Curation, Visualization, Writing—original draft, Funding acquisition. AH: Supervision, Resources, Project administration, Formal analysis, Data Curation, Funding acquisition. GG: Supervision, Conceptualization, Resources, Writing—original draft, Writing—review & editing, Funding acquisition.
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Dutta, D., Harper, A. & Gangopadhyay, G. Transcriptomic analysis of high oil-yielding cultivated white sesame and low oil-yielding wild black sesame seeds reveal differentially expressed genes for oil and seed coat colour. Nucleus 65, 151–164 (2022). https://doi.org/10.1007/s13237-022-00389-0
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DOI: https://doi.org/10.1007/s13237-022-00389-0