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Assessment of stability and viability of a jute hybrid (Corchorus capsularis L. × C. trilocularis L.) and its trueness based on RAPD markers

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Abstract

A hybrid line (Corchorus capsularis L. – pollen parent × C. trilocularis L. – stigma parent) of jute (family: Tiliaceae) has been assessed for its stability (using cytomorphological parameters) and viability (using different stain tests including DAPI - 4′,6-diamidino-2-phenylindole) in advance generations (F4 to F6). The trueness of the hybrid is studied following the use of RAPD (random amplification of polymorphic DNA) markers. Results indicate that the hybrid line is stable, viable and true. UPGMA (Unweighted Pair Group Method with Arithmetic Mean) analysis suggests a close relationship as well as distinctiveness between parents and the raised hybrid. Upon considering different genetic efficiency parameters, taken together, it seems that OPA 02, OPA 03, OPB 01, OPB 02, OPB 06, OPC 01 and OPC 06 are effective and efficient RAPD markers for molecular screening of jute germplasms including hybrid(s). OPB 06 is found to be female specific RAPD marker.

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References

  1. Ali MA, Seyal MT, Awan SI, Niaz S, Ali S, Abbas A. Hybrid authentication in upland cotton through RAPD analysis. Aust J Crop Sci. 2008;2:141–9.

    CAS  Google Scholar 

  2. Anderson JA, Churchill GA, Autrique JE, Tanksley SD, Sorrells ME. Optimizing parental selection for genetic linkage maps. Genome. 1993;36:181–6.

    Article  CAS  PubMed  Google Scholar 

  3. Arangzeb S. Cross compatibility of eight wild species of jute with cultivars and among themselves. Ph.D. thesis, University of Dhaka, Dhaka, Bangladesh; 1994.

  4. Arangzeb S, Khatun A. A short note on interspecific hybridization between C. trilocularis and C. capsularis. Bangladesh J Jute Fibre Res. 1980;5:85–9.

    Google Scholar 

  5. Atiaksheva LF, Pilipenko OS, Poltorak OM. Mechanism of the thermoinactivation of the β-galactosidase from Escherichia coli. In: International Symposium, Biocatalysis-2000: Fundamentals & Applications, Lomonosov Moscow State University, Khimiya, Moscow, Russia. 2000;41(No.6. Supplement):95–7. www.chem.msu.su/eng/journals/vmgu/00add/95.pdf. Accessed 01 July 2013.

  6. Bengtsson S. Evaluation of transgenic Campanula carpatica plants. In: Epsilon Undergraduate Theses Archive: vol. 6. Dept. of Agricultural Sciences, The Royal Veterinary and Agricultural University; 2006. http://ex-epsilon.slu.se:8080/archive/00001970/. Accessed 01 July 2013.

  7. Bolat I, Pirlak L. An investigation on pollen viability, germination and tube growth in some stone fruits. Turk J Agric For. 1999;23:383–8.

    Google Scholar 

  8. Bots M, Mariani C. Pollen viability in the field. In: COGEM report, Radboud Universiteit Nijmegen, Netherlands; 2005. http://www.cogem.net/showdownload.cfm?objectId=FFFE6554-1517-64D9-CC081ED9E42EF28C&objectType=mark.apps.cogem.contentobjects.publication.download.pdf. Accessed 01 July 2013.

  9. Chaudhuri SD, Mia AJ. Species crosses in the genus Corchorus (jute plants). Euphytica. 1962;11:61–4.

    Article  Google Scholar 

  10. Datta RM, Banerji SN. On the cause of seed failure or seed setting in 4n cultivated Corchorus capsularis L. x 2n cultivated C. capsularis and 4n cultivated C. olitorius L. x 2n cultivated C. olitorius and their reciprocals through cytogenetical studies. Genetica. 1960;31:385–409.

    Article  CAS  PubMed  Google Scholar 

  11. Datta RM, Choudhury MR. Seed failure following hybridization between 4n Corchorus capsularis L. (strain D. 154) and 3n C. olltorius L. (strain C. G.) and its reciprocal and some cytogenetical observations. Biol Plant Acad Sci Bohemoslov. 1966;8:288–91.

    Google Scholar 

  12. Farzaneh T, Sheidai M, Nourmohammadi Z, Alishah O, Farahani F. Cytogenetic and RAPD analysis of cotton cultivars and their F1 progenies. Caryologia. 2010;63:73–81.

    Google Scholar 

  13. Ferri A, Giordani E, Padula G, Bellini E. Viability and in vitro germinability of pollen grains of olive cultivars and advanced selections obtained in Italy. Adv Hortic Sci. 2008;22:116–22.

    Google Scholar 

  14. Haiyuan D, Gengyun Z, Yan G, Shaolin C, Shouyi C. RAPD tagging of salt tolerant genes in rice. Chin Sci Bull. 1998;43:330–2.

    Article  Google Scholar 

  15. Haque M, Islam AS. Some promising material among F4 and back-cross derivatives of the natural hybrid C. aestuans × C. olitorius. Sind Univ Sci Res J. 1970;4:97–107.

    Google Scholar 

  16. Islam AS, Sattar MA. Interspecific hybridization in the genus Corchorus: C. aestuans X C. capsularis. In: Proceeding XIII Pakistan Science Conference, Dacca, Pakistan, Dacca; 1961. p. 6–7.

  17. Iva B, Snezana M, Milomir F, Kosana K. Genetic characterization of early maturing maize hybrids (Zea mays L.) obtained by protein and RAPD markers. Acta Biol Iugosl. 2005;37:235–43.

    Google Scholar 

  18. Johnson S, McCormick S. Pollen germinates precociously in the anthers of raring-to-go, an Arabidopsis thaliana gametophytic mutant. Plant Physiol. 2001;126:685–95.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Maity S, Datta AK. Cytomorphological studies in F1 hybrids (Corchorus capsularis L. x Corchorus olitorius L.) of jute (Tiliaceae). Comp Cytogenet. 2008;2:143–9.

    Google Scholar 

  20. Maity S, Datta AK. Cytomorphological studies in F 2, F3 and induced amphidiploid of jute (Corchorus trilocularis L. X Corchorus capsularis L.). Nucleus. 2010;53:85–7.

    Article  Google Scholar 

  21. Mandal A, Datta AK, Bhattacharya A. Evaluation of pollen and productive parameters, their interrelationship and clustering of eight Corchorus spp. (Tiliaceae). Nucleus. 2011;54:147–52.

    Article  Google Scholar 

  22. Mandal A, Datta AK, Datta S, Gupta S. Genetic assessment of eight Corchorus spp. (Tiliaceae) using RAPD and ISSR markers. Nucleus. 2013;56:23–30.

    Article  Google Scholar 

  23. Marks GE. An aceto-carmine glycerol jelly for use in pollen-fertility counts. Biotechnic Histochem. 1954;29:277–8.

    Article  CAS  Google Scholar 

  24. Martynov SP, Dobrotvorskaya TV, Dotlacil L, Stehno Z, Faberova I, Bares I. Genealogical approach to the formation of the winter wheat core collection. Russ J Genet. 2003;39:917–23.

    Article  CAS  Google Scholar 

  25. Mehetre SS, Aher AR, Shinde GC, Gomes M, Eapen S. RAPD analysis of interspecific hybrid between Gossypium arboreum and Gossypium stocksii. Caryologia. 2004;57:167–71.

    Article  Google Scholar 

  26. Nayeem KA, Dalvi DG. A rapid technique for obtaining leaf prints for stomatal count with Fevicol. Curr Sci. 1989;58:640–1.

    Google Scholar 

  27. Patil VN, Dadlani M. “Tetrazolium test for seed viability and vigour”. In: Handbook of Seed Testing. 2009. p. 209–41.

  28. Regan SM, Moffatt BA. Cytochemical analysis of pollen development in wild-type Arabidopsis and a male-sterile mutant. Plant Cell. 1990;2:877–89.

    CAS  PubMed Central  PubMed  Google Scholar 

  29. Smith JF, Burke CC, Wagner WL. Interspecific hybridization in natural populations of Cyrtandra (Gesneriaceae) on the Hawaiian Islands: evidence from RAPD markers. Pl Syst Evol. 1996;200:61–77.

    Article  CAS  Google Scholar 

  30. Swaminathan MS, Iyer RD. Skewed recombination in a rare interspecific jute hybrid. Nature. 1961;192:893–4.

    Article  Google Scholar 

  31. Weir BS. Genetic data analysis II: methods for discrete population genetic data. Sunderland: Sinauer Publishers; 1996.

    Google Scholar 

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Acknowledgment

The aided research grant by University Grants Commission (UGC), New Delhi, India is thankfully acknowledged.

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Authors and Affiliations

  1. Department of Botany, Cytogenetics, Genetics and Plant Breeding Section, University of Kalyani, Kalyani, 741235, West Bengal, India

    Aninda Mandal, Animesh K. Datta & Benoy K. Ghosh

  2. Department of Botany, Pteridology-Palaeobotany Section, University of Kalyani, Kalyani, 741235, West Bengal, India

    Sudha Gupta

  3. Department of Biotechnology, Haldia Institute of Technology, Haldia, 721657, West Bengal, India

    Siraj Datta

  4. P.G. Department of Botany, Hooghly Mohsin College, Hooghly, 712101, West Bengal, India

    Sonali Sengupta

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  1. Aninda Mandal
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  2. Animesh K. Datta
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Correspondence to Animesh K. Datta.

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Mandal, A., Datta, A.K., Datta, S. et al. Assessment of stability and viability of a jute hybrid (Corchorus capsularis L. × C. trilocularis L.) and its trueness based on RAPD markers. Nucleus 56, 163–170 (2013). https://doi.org/10.1007/s13237-013-0093-5

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  • DOI: https://doi.org/10.1007/s13237-013-0093-5

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