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Effect of stromal cell derived factor-1α release from heparin-coated Co-Cr stent substrate on the recruitment of endothelial progenitor cells

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Abstract

The restoration of a damaged endothelium might be a fascinating way to reduce significantly late-thrombosis and restenosis in the stent treatment. It has been recently reported that the recruitment of endothelial progenitor cells (EPCs), capable for repairing a damaged endothelium, can be important in the vascular stent application. Therefore, we focused on the hypothesis that stromal cell-derived factor-1α (SDF-1α) acts as a key chemokine for the mobilization and recruitment of EPCs to the damaged endothelium lesion. In this study, the effect of SDF-1α released from a cobalt-chromium alloy (Co-Cr) plate, vascular stent material, was investigated on the recruitment of EPCs in vitro. Dopamine-conjugated heparin was synthesized to introduce heparin onto Co-Cr. Heparin-coated Co-Cr surfaces were examined by water contact angle and attenuated total reflectance-Fourier transform infrared (ATRFTIR) to prove successful coating of heparin. Finally, SDF-1α was bound to the coated heparin derivative. Amounts of loaded and released SDF-1α were measured by ELISA, and the recruited EPC by released SDF-1α was evaluated using two different migration assays. The quantity of SDF-1α bound to the heparin-modified surface increased in a concentration-dependent manner and SDF-1α was released over 28 days. Transwell migration assay revealed that soluble SDF-1α released from the heparin-coated Co-Cr induced significantly more EPC recruitment than bare Co-Cr and heparin-coated Co-Cr. More importantly, fibrin gel migration assay further demonstrated that EPCs evidently respond to heparin-based substrate with SDF-1α and this type of behaviors was surprisingly found at as low level as less 1 ng/day of SDF-1α. These results are strongly encouraging for further in vitro and in vivo studies.

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References

  1. V. L. Roger, A. S. Go, D. M. Lloyd-Jones, E. J. Benjamin, J. D. Berry, W. B. Borden, D. M. Bravata, S. Dai, E. S. Ford, C. S. Fox, H. J. Fullerton, C. Gillespie, S. M. Hailpern, J. A. Heit, V. J. Howard, B. M. Kissela, S. J. Kittner, D. T. Lackland, J. H. Lichtman, L. D. Lisabeth, D. M. Makuc, G. M. Marcus, A. Marelli, D. B. Matchar, C. S. Moy, D. Mozaffarian, M. E. Mussolino, G. Nichol, N. P. Paynter, E. Z. Soliman, P. D. Sorlie, N. Sotoodehnia, T. N. Turan, S. S. Virani, N. D. Wong, D. Woo, M. B. Turner, C. American Heart Association Statistics, and S. Stroke Statistics, Circulation, 125, 188 (2012).

    Article  Google Scholar 

  2. J. W. Moses, M. B. Leon, J. J. Popma, P. J. Fitzgerald, D. R. Holmes, C. O’Shaughnessy, R. P. Caputo, D. J. Kereiakes, D. O. Williams, P. S. Teirstein, J. L. Jaeger, R. E. Kuntz, and S. Investigators, N. Engl. J. Med., 349, 1315 (2003).

    Article  CAS  Google Scholar 

  3. S. Ahn, J.-S. Sung, B. Choi, H. Kim, and Y. Sung, Biomater. Res., 18, 17 (2014).

    Article  Google Scholar 

  4. M. Joner, A. V. Finn, A. Farb, E. K. Mont, F. D. Kolodgie, E. Ladich, R. Kutys, K. Skorija, H. K. Gold, and R. Virmani, J. Am. Coll. Cardiol., 48, 193 (2006).

    Article  Google Scholar 

  5. E. Seo and K. Na, Biomater. Res., 18, 15 (2014).

    Article  Google Scholar 

  6. T. M. Bedair, Y. Cho, T. J. Kim, Y. D. Kim, B. J. Park, Y. K. Joung, and D. K. Han, Langmuir, 30, 8020 (2014).

    Article  CAS  Google Scholar 

  7. S. N. Kang, S.-E. Kim, J. Choi, K. Park, J. H. Goo, D. S. Sim, Y. J. Hong, J. H. Kim, Y. K. Joung, J. Lee, M. H. Jeong, and D. K. Han, Biomaterials, 44, 1 (2015).

    Article  CAS  Google Scholar 

  8. W.-H. Lim, W.-W. Seo, W. Choe, C.-K. Kang, J. Park, H.-J. Cho, S. Kyeong, J. Hur, H.-M. Yang, H.-J. Cho, Y.-S. Lee, and H.-S. Kim, Arterioscler. Thromb. Vasc. Biol., 31, 2798 (2011).

    Article  CAS  Google Scholar 

  9. T. Asahara, T. Murohara, A. Sullivan, M. Silver, R. van der Zee, T. Li, B. Witzenbichler, G. Schatteman, and J. M. Isner, Science, 275, 964 (1997).

    Article  CAS  Google Scholar 

  10. N. Werner, S. Junk, U. Laufs, A. Link, K. Walenta, M. Böhm, and G. Nickenig, Circ. Res., 93, e17 (2003).

    Article  CAS  Google Scholar 

  11. M. Hristov and C. Weber, Pharmacol. Res., 58, 148 (2008).

    Article  CAS  Google Scholar 

  12. J. D. Abbott, Y. Huang, D. Liu, R. Hickey, D. S. Krause, and F. J. Giordano, Circulation, 110, 3300 (2004).

    Article  Google Scholar 

  13. C. Urbich, E. Chavakis, and S. Dimmeler, in Tumor Angiogenesis, D. Marmé and N. Fusenig, Eds., Springer, Berlin Heidelberg, 2008, pp 309–324.

  14. T. T. Lau and D.-A. Wang, Expert Opin. Biol. Ther., 11, 189 (2011).

    Article  CAS  Google Scholar 

  15. K.-W. Lee, N. R. Johnson, J. Gao, and Y. Wang, Biomaterials, 34, 9877 (2013).

    Article  CAS  Google Scholar 

  16. L. Baumann, S. Prokoph, C. Gabriel, U. Freudenberg, C. Werner, and A. G. Beck-Sickinger, J. Control. Release, 162, 68 (2012).

    Article  CAS  Google Scholar 

  17. B. P. Purcell, J. A. Elser, A. Mu, K. B. Margulies, and J. A. Burdick, Biomaterials, 33, 7849 (2012).

    Article  CAS  Google Scholar 

  18. S. Bhakta, P. Hong, and O. Koc, Cardiovasc. Revasc. Med., 7, 19 (2006).

    Article  Google Scholar 

  19. J. Yu, A. Wang, Z. Tang, J. Henry, B. Lee, Y. Zhu, F. Yuan, F. Huang, and S. Li, Biomaterials, 33, 8062 (2012).

    Article  CAS  Google Scholar 

  20. J.-B. Huh, J.-Y. Lee, K.-L. Lee, S.-E. Kim, M.-J. Yun, J.-S. Shim, J.-S. Shim, and S.-W. Shin, J. Adv. Prosthodont., 3, 152 (2011).

    Article  Google Scholar 

  21. K. S. Jee, H. D. Park, K. D. Park, Y. H. Kim, and J.-W. Shin, Biomacromolecules, 5, 1877 (2004).

    Article  CAS  Google Scholar 

  22. I. You, S. M. Kang, Y. Byun, and H. Lee, Bioconjug. Chem., 22, 1264 (2011).

    Article  CAS  Google Scholar 

  23. N. Yamaguchi, L. Zhang, B.-S. Chae, C. S. Palla, E. M. Furst, and K. L. Kiick, J. Am. Chem. Soc., 129, 3040 (2007).

    Article  CAS  Google Scholar 

  24. L. Ye, X. Wu, H.-Y. Duan, X. Geng, B. Chen, Y.-Q. Gu, A.-Y. Zhang, J. Zhang, and Z.-G. Feng, J. Biomed. Mater. Res. A, 100, 3251 (2012).

    Article  Google Scholar 

  25. L. Ye, X. Wu, Q. Mu, B. Chen, Y. Duan, X. Geng, Y. Gu, A. Zhang, J. Zhang, and Z.-G. Feng, J. Biomater. Sci. Polym. Ed., 22, 389 (2011).

    Article  CAS  Google Scholar 

  26. H. Lee, S. M. Dellatore, W. M. Miller, and P. B. Messersmith, Science, 318, 426 (2007).

    Article  CAS  Google Scholar 

  27. I.-H. Bae, I.-K. Park, D. Park, H. Lee, and M. Jeong, J. Mater. Sci., Mater. Med., 23, 1259 (2012).

    Article  CAS  Google Scholar 

  28. J.-H. Jiang, L.-P. Zhu, X.-L. Li, Y.-Y. Xu, and B.-K. Zhu, J. Membr. Sci., 364, 194 (2010).

    Article  CAS  Google Scholar 

  29. H. Lee, J. Rho, and P. B. Messersmith, Adv. Mater., 21, 431 (2009).

    Article  CAS  Google Scholar 

  30. L. Ma, H. Qin, C. Cheng, Y. Xia, C. He, C. Nie, L. Wang, and C. Zhao, J. Mater. Chem. B, 2, 363 (2014).

    Article  CAS  Google Scholar 

  31. Y. S. Jung, J.-H. Jeong, S. Yook, B.-H. Im, J. Seo, S. W. Hong, J.-B. Park, V. C. Yang, D. Y. Lee, and Y. Byun, Biomaterials, 33, 295 (2012).

    Article  CAS  Google Scholar 

  32. S. Prokoph, E. Chavakis, K. R. Levental, A. Zieris, U. Freudenberg, S. Dimmeler, and C. Werner, Biomaterials, 33, 4792 (2012).

    Article  CAS  Google Scholar 

  33. A. Amara, O. Lorthioir, A. Valenzuela, A. Magerus, M. Thelen, M. Montes, J.-L. Virelizier, M. Delepierre, F. Baleux, H. Lortat- Jacob, and F. Arenzana-Seisdedos, J. Biol. Chem., 274, 23916 (1999).

    Article  CAS  Google Scholar 

  34. R. Sadir, F. Baleux, A. Grosdidier, A. Imberty, and H. Lortat-Jacob, J. Biol. Chem., 276, 8288 (2001).

    Article  CAS  Google Scholar 

  35. Z. Yang, Q. Tu, J. Wang, and N. Huang, Biomaterials, 33, 6615 (2012).

    Article  CAS  Google Scholar 

  36. A. Gardais, J. Picard, and B. Hermelin, Comp. Biochem. Phys. B, 44, 507 (1973).

    CAS  Google Scholar 

  37. L. Kjellen, A. Oldberg, K. Rubin, and M. Hook, Biochem. Biophys. Res. Commun., 74, 126 (1977).

    Article  CAS  Google Scholar 

  38. H. Rubin, Nat. Med., 2, 632 (1996).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Korea

    Sung Nam Kang, Chungwon Park, Seong Min Kim, Ki Wan Park, Dong Keun Han & Yoon Ki Joung

  2. Department of Biomedical Engineering, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Korea

    Sung Nam Kang, Chungwon Park, Dong Keun Han & Yoon Ki Joung

  3. School of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea

    Seong Min Kim

  4. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 04107, Korea

    Ki Wan Park

  5. Department of Electronic Engineering and Institute of Gachon Fusion Technology, Gachon University, Seongnam, Gyeonggi, 13120, Korea

    Bang Ju Park

Authors
  1. Sung Nam Kang
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  2. Chungwon Park
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  4. Ki Wan Park
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  7. Yoon Ki Joung
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Correspondence to Dong Keun Han or Yoon Ki Joung.

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Kang, S.N., Park, C., Kim, S.M. et al. Effect of stromal cell derived factor-1α release from heparin-coated Co-Cr stent substrate on the recruitment of endothelial progenitor cells. Macromol. Res. 23, 1159–1167 (2015). https://doi.org/10.1007/s13233-015-4002-z

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  • DOI: https://doi.org/10.1007/s13233-015-4002-z

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