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Absorption of Casein Antihypertensive Peptides through an In Vitro Model of Intestinal Epithelium

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Food Digestion

Abstract

A commercial casein hydrolysate, which contains peptides RYLGY and AYFYPEL as active molecules, has shown antihypertensive effects after acute and long-term administration. This study examines transepithelial absorption of RYLGY and AYFYPEL and derived digestion fragments using Caco-2, HT29-MTX−6 and co-culture 75 % Caco-2/25 % HT29-MTX−6 as predictive models and RP-HPLC–MS as analytical tool. Peptides RYLGY and AYFYPEL were absorbed intact through cell monolayers, although RYLGY was partly hydrolyzed by intestinal peptidases. Co-culture 75 % Caco-2/25 % HT29-MTX−6 exhibited intermediate properties, with regard to transepithelial electrical resistance, peptide hydrolysis, and absorption of the studied peptides, between Caco-2 and HT29-MTX−6 pure cultures. Interestingly, mucus layer that covered completely HT29-MTX−6 and co-culture monolayers was not a barrier for the absorption of the studied peptides. The apparent permeability values for absorptive transport across Caco-2 monolayers for RYLGY (0.22 × 10−6 cm/s) and AYFYPEL (0.26 × 10−6 cm/s) were similar. These findings highlight that in vivo absorption of antihypertensive peptides RYLGY and AYFYPEL may occur partially as intact peptides.

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Acknowledgments

This work has received financial support from the projects AGL2011-24643 and Consolider Ingenio 2010 FUN-C-Food CSD2007-00063 both from the Ministry of Economy and Competitivenes, and COST Action oc-2010-1-7087 (INFOGEST).

Conflict of Interest

The authors report no conflict of interest.

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Author notes

  1. María del Mar Contreras

    Present address: Faculty of Sciences, Department of Analytical Chemistry, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain

  2. Ana I. Sancho

    Present address: Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020, Salzburg, Austria

  3. Clare Mills

    Present address: Manchester Interdisciplinary Biocentre, Manchester Academic Health Science Centre, School of Translational Medicine, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK

Authors and Affiliations

  1. Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM), Nicolás Cabrera, 9. Campus de Cantoblanco, Madrid, 28049, Spain

    María del Mar Contreras & Isidra Recio

  2. Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK

    Ana I. Sancho & Clare Mills

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  1. María del Mar Contreras
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Correspondence to Isidra Recio.

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del Mar Contreras, M., Sancho, A.I., Recio, I. et al. Absorption of Casein Antihypertensive Peptides through an In Vitro Model of Intestinal Epithelium. Food Dig. 3, 16–24 (2012). https://doi.org/10.1007/s13228-012-0020-2

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  • DOI: https://doi.org/10.1007/s13228-012-0020-2

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