Skip to main content
SpringerLink
Log in

An emended description of Fusarium brevicatenulatum and F. pseudoanthophilum based on isolates recovered from millet in Kenya

  • Published:
Fungal Diversity Aims and scope Submit manuscript

Abstract

Cultures morphologically similar to F. brevicatenulatum and F. pseudoanthophilum were recovered from millet collected in Kenya. Morphological characterisation showed that the production of pyriform microconidia and chlamydospores, key characters separating these species from each other and from other Fusarium species, was variable in these cultures. Sexual compatibility studies of these isolates and holotype cultures of F. brevicatenulatum and F. pseudoanthophilum revealed that they belonged to the same biological species. No fertile crosses were observed between putative isolates of F. brevicatenulatum from millet and tester strains of F. verticillioides, F. sacchari, F. proliferatum, F. subglutinans, F. thapsinum, and F. konzum. Neither were fertile crosses obtained from any self-cross tests, indicating that the cultures were heterothallic. Further studies using AFLP, and sequencing of the β-tubulin, calmodulin, and translation elongation factor 1-α genes revealed that the isolates from the millets and those of the ex-holotype cultures of F. brevicatenulatum and F. pseudoanthophilum belonged to the same species. The species epithet F. brevicatenulatum is preferred on the basis of priority.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Britz H, Wingfield MJ, Coutinho TA, Marasas WFO, Leslie JF (1998) Female fertility and mating type distribution in a South African population of Fusarium subglutinans f.sp. pini. Appl Environ Microbiol 64:2094–2095

    CAS  PubMed  Google Scholar 

  • Britz H, Coutinho TA, Wingfield MJ, Marasas WFO, Gordon TR, Leslie JF (1999) Fusarium subglutinans f. sp. pini represents a distinct mating population in the Gibberella fujikuroi species complex. Appl Environ Microbiol 65:1198–1201

    CAS  PubMed  Google Scholar 

  • Britz H, Coutinho TA, Wingfield MJ, Marasas WFO (2002) Validation of the description of Gibberella circinata and morphological differentiation of the anamorph Fusarium circinatum. Sydowia 54:9–22

    Google Scholar 

  • Bullock S, Willetts HJ, Ashford AE (1980) The structure and histochemistry of sclerotia of Sclerotinia minor Jagger 1. Light and electron microscope studies on sclerotial development. Protoplasma 31:5–331

    Google Scholar 

  • Burgess LW, Summerell BA, Bullock S, Gott KP, Backhouse D (1994) Laboratory manual for Fusarium research. University of Sydney, Fusarium Research Laboratory

    Google Scholar 

  • Chulze SN, Ramirez ML, Torres A, Leslie JF (2000) Genetic variation in Fusarium section Liseola from no-till maize in Argentina. Appl Environ Microbiol 66:5312–5315

    Article  CAS  PubMed  Google Scholar 

  • Dice LR (1945) Measures of the amount of ecological association between species. Ecology 26:297–302

    Article  Google Scholar 

  • Donoghue M (1985) A critique of the biological species concept and recommendations for a phylogenetic alternative. Bryologist 88:172–181

    Article  Google Scholar 

  • Feder N, O’Brien P (1968) Plant microtechnique: some principles and new methods. Am J Bot 55:123–142

    Article  Google Scholar 

  • Fisher NL, Burgess LW, Toussoun TA, Nelson PE (1982) Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology 72:151–153

    Article  Google Scholar 

  • Hasegawa M, Kishino K, Yano TA (1985) Dating of human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 21:160–174

    Article  Google Scholar 

  • Hsieh WH, Smith SN, Snyder WC (1977) Mating groups in Fusarium moniliforme. Phytopathology 67:1041–1043

    Article  Google Scholar 

  • Kerényi Z, Zeller KA, Hornok L, Leslie JF (1999) Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex. Appl Environ Microbiol 65:4071–4076

    PubMed  Google Scholar 

  • Klittich CJR, Leslie JF (1992) Identification of a second mating population within the Fusarium moniliforme anamorph of Gibberella fujikuroi. Mycologia 84:541–547

    Article  Google Scholar 

  • Kornerup A, Wanscher JH (1963) Methuen handbook of colour. Methuen and Co, Ltd

    Google Scholar 

  • Kuhlman EG (1982) Varieties of Gibberella fujikuroi with anamorphs in Fusarium section Liseola. Mycologia 74:759–768

    Article  Google Scholar 

  • Leslie JF (1991) Mating populations in Gibberella fujikuroi (Fusarium section Liseola). Phytopathology 81:1058–1060

    Google Scholar 

  • Leslie JF, Klein KK (1996) Female fertility and mating type effects on effective population size and evolution in filamentous fungi. Genetics 144:557–567

    CAS  PubMed  Google Scholar 

  • Leslie JF, Marasas WFO (2002) Will the real “Fusarium moniliforme” please stand up! In: Leslie JF (ed) Sorghum and Millets Diseases. Iowa State Press, Blackwell Publishing Company

  • Leslie JF, Zeller KA, Summerell BA (2001) Icebergs and species in populations of Fusarium. Physiol Mol Plant Pathol 59:107–117

    Article  Google Scholar 

  • Leslie JF, Zeller K, Wohler M, Summerell BA (2004) Interfertility of two mating populations in the Gibberella fujikuroi species complex. Eur J Plant Pathol 110:611–618

    Article  CAS  Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

    Article  CAS  PubMed  Google Scholar 

  • Nirenberg H (1976) Studies on the morphological and biological differentiation in the Fusarium- section Liseola. Reports from Federal Centre of Biology for Agriculture and Forestry. Berlin - Dahlem. pp 2–110

  • Nirenberg HI, O’Donnell K (1998) New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434–458

    Article  Google Scholar 

  • Nirenberg HI, Kroschel J, Adrianaivo AP, Frank JM, Mubatanhema W (1998) Two new species of Fusarium: Fusarium brevicatenulatum from noxious weed Striga asiatica in Madagascar and Fusarium pseudoanthophilum from Zea mays in Zimbabwe. Mycologia 90:459–464

    Article  Google Scholar 

  • O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116

    Article  PubMed  Google Scholar 

  • O’Donnell K, Cigelnik E, Nirenberg H (1998) Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90:465–493

    Article  Google Scholar 

  • O’Donnell K, Nirenberg HI, Aoki T, Cigelnik E (2000) A multigene phylogeny of the Gibberella fujikuroi species complex: Detection of additional phylogenetically distinct species. Mycoscience 41:61–78

    Article  Google Scholar 

  • Phan HT, Burgess LW, Summerell BA, Bullock S, Liew ECY, Smith-White JL, Clarkson JR (2004) Gibberella gaditjirrii(Fusarium gaditjirrii) sp. nov., a new species from tropical grasses in Australia. Stud Mycol 50:261–272

    Google Scholar 

  • Steenkamp E, Wingfield BD, Coutinho TA, Wingfield MJ, Marasas WFO (2000) PCR-based identification of MAT-1 and MAT-2 in the Gibberella fujikuroi species complex. Appl Environ Microbiol 66:4378–4382

    Article  CAS  PubMed  Google Scholar 

  • Summerell BA, Salleh B, Leslie JF (2003) A utilitarian approach to Fusarium identification. Plant Dis 87:117–128

    Article  Google Scholar 

  • Swofford DL (1998) PAUP*. Phylogenetic Analysis Using Parsimony (*and their methods). Version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS, Fisher MC (2000) Phylogenetic species recognition and species concepts in Fungi. Fungal Genet Biol 31:21–32

    Article  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4822

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  CAS  PubMed  Google Scholar 

  • Zeller KA, Summerell BA, Bullock S, Leslie JF (2003) Gibberella konza (Fusarium konzum) sp. nov. from prairie grasses, a new species in the Gibberella fujikuroi species complex. Mycologia 95:943–954

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kenya Agricultural Research Institute/National Agricultural Research Laboratories, P.O.Box 14733, 00800, Nairobi, Kenya

    R. L. Amata

  2. Faculty of Agriculture Food and Natural Resources, University of Sydney, Sydney, NSW, 2006, Australia

    L. W. Burgess & J. L. Smith-White

  3. Royal Botanic Gardens and Domains Trust, Mrs. Macquaries Road, Sydney, NSW, 2000, Australia

    B. A. Summerell, S. Bullock & E. C. Y. Liew

Authors
  1. R. L. Amata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. W. Burgess
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. A. Summerell
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Bullock
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. C. Y. Liew
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. L. Smith-White
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. A. Summerell.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Amata, R.L., Burgess, L.W., Summerell, B.A. et al. An emended description of Fusarium brevicatenulatum and F. pseudoanthophilum based on isolates recovered from millet in Kenya. Fungal Diversity 43, 11–25 (2010). https://doi.org/10.1007/s13225-010-0019-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13225-010-0019-3

Keywords

Search

Navigation