Abstract
The lactic acid bacterium Pediococcus acidilactici has recently been reported to help in treating constipation, diarrhea, relieving stress, and enhancing growth rate and immune response in humans, birds, fishes, and small animals. In the present study, we sequenced and analyzed the whole genome of P. acidilactici NRCC1, a novel isolate from rumen fluid of dromedary camel (Camelus dromedarius). The genome of P. acidilactici NRCC1 was assembled into 60 contigs, comprising 1,785,679 bp and 42.5% GC content. The 1705 CDS were predicted and annotated using the RAST server. The genome encodes numerous enzymes for utilization of different carbohydrates. It also harbors genes for antibiotic biosynthesis and many others which might confer probiotic properties. The comparative genome analysis with P. acidilactici DSM 20284 revealed some unique features in P. acidilactici NRCC1. Thus, the genome sequencing of P. acidilactici NRCC1 has opened up new horizons for further research in animal probiotics and feed supplements.
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Acknowledgements
The authors are thankful to Ankit T Hinsu and Ravi Shah, College of Veterinary Science and Animal Husbandry, Anand Agricultural University (AAU), Anand, Gujarat for extending their technical support in the sequencing and Kashinath, Senior Technical Officer, ICAR-NRCC, Bikaner for the help in rumen fluid collection.
Funding
This work is supported by a grant from ICAR-National Centre for Veterinary Type Culture Collection, National Research Centre on Equines, Hisar, Haryana, India under Veterinary Type Culture Collection-Rumen Microbe project.
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Ranjan, R., Pandit, R.J., Duggirala, S.M. et al. Genome sequencing of Pediococcus acidilactici (NRCC1), a novel isolate from dromedary camel (Camelus dromedarius) rumen fluid. Ann Microbiol 68, 103–110 (2018). https://doi.org/10.1007/s13213-017-1320-0
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DOI: https://doi.org/10.1007/s13213-017-1320-0