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Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product “Btana”

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Abstract

In this study, using high throughput “pyrosequencing,” we highlighted the bacterial diversity of the traditional Algerian date product “Btana” that is produced in southern Algeria using both direct (DBM) and indirect (UBM) methods. Metagenomic analysis yielded a total of 103,379 reads, with a 606 total operational taxonomic units (OTUs) detected. Firmicutes represented 84.79 % of the total pyrosequencing reads. Phylogenetic analysis revealed that the Bacillales represented 90.20 % ± 15.12 % of the total reads. Among the phylotypes detected, Bacillus was the dominant genus (39.53 %). While Bacillus megaterium was shared among all of the samples, its distribution varied widely. Non-metric multidimensional scaling (nMDS) analysis showed that UBM samples clustered together, and three main OTUs were found in these UBM samples: Paenibacillus polymyxa, Paenibacillus xylanexedens, and Planomicrobium JN082684. Correlation analysis showed no association between parameters of the samples (age, pH, water activity) and the specific microbiota.

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Authors and Affiliations

  1. Department of Food Sciences, Faculty of Veterinary Medicine, Fundamental and Applied Research on Animal and Health (FARAH), University of Liège, Sart-Tilman, 4000, Liege, Belgium

    Abdelkader Abekhti, Bernard Taminiau & Georges Daube

  2. Department of Biology, Faculty of Science, Laboratory of Applied Microbiology, Oran University, BP16, Es-senia, 31000, Oran, Algeria

    Abdelkader Abekhti & Mabrouk Kihal

  3. Department of Natural and Life Sciences, Faculty of Exact Sciences and Natural and Life Sciences, University of Biskra, BP 145, 07000, Biskra, Algeria

    Abdelkader Abekhti

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  1. Abdelkader Abekhti
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  2. Bernard Taminiau
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  3. Mabrouk Kihal
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  4. Georges Daube
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Correspondence to Abdelkader Abekhti.

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Abekhti, A., Taminiau, B., Kihal, M. et al. Metagenomic analysis of the bacterial microbiota linked to the traditional Algerian date product “Btana”. Ann Microbiol 65, 2415–2424 (2015). https://doi.org/10.1007/s13213-015-1084-3

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