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Production of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114

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Abstract

An actinobacteria strain PAL114, isolated from a Saharan soil in Algeria, produces bioactive compounds. Morphological and chemical studies indicated that this strain belongs to the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.8 % with S. griseoflavus LMG 19344T, the most closely related species. Two bioactive compounds, named P44 and P40, were extracted by dichloromethane from the cell-free supernatant broth and were purified by HPLC. Minimum inhibitory concentrations (MIC) of the compounds were determined against pathogenic and toxigenic microorganisms, most of which are multiresistant to antibiotics. The P40 fraction showed a strong activity especially against Candida albicans, Bacillus subtilis, and Staphylococcus aureus and has lower MIC values than those of P44 against most microorganisms tested. Chemical structures of compounds were determined based on spectroscopic and spectrometric analyses (UV-visible, mass, 1H, and 13C NMR spectra). The compounds P44 and P40 were identified as vineomycin A1 and chaetoglobosin A, respectively. Vineomycin A1 is known to be produced by some Streptomyces species. However, chaetoglobosin A is known to be produced only by fungi belonging to the genera Chaetomium, Penicillium, and Calonectria. This is the first time that chaetoglobosin A, known for its antimicrobial, anticancer, and cytotoxic effects, is reported in prokaryotes.

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Authors and Affiliations

  1. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria

    Adel Aouiche, Atika Meklat, Abdelghani Zitouni & Nasserdine Sabaou

  2. Laboratoire de Chimie de Coordination (LCC), CNRS, UPS, INPT, LCC, Université de Toulouse, 205, Route de Narbonne, 31077, Toulouse, France

    Christian Bijani

  3. Université de Toulouse, laboratoire de génie chimique UMR 5503 (CNRS/INPT/UPS), INP de Toulouse/ENSAT, 1 Avenue de l’Agrobiopôle, Castanet-Tolosan cedex, France

    Florence Mathieu

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  1. Adel Aouiche
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  2. Atika Meklat
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  3. Christian Bijani
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  4. Abdelghani Zitouni
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  5. Nasserdine Sabaou
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  6. Florence Mathieu
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Correspondence to Nasserdine Sabaou or Florence Mathieu.

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Aouiche, A., Meklat, A., Bijani, C. et al. Production of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114. Ann Microbiol 65, 1351–1359 (2015). https://doi.org/10.1007/s13213-014-0973-1

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  • DOI: https://doi.org/10.1007/s13213-014-0973-1

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