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Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch

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Abstract

This study reports enhanced degradation of crude oil hydrocarbons by a bacterial consortium culture (CC) immobilized onto sawdust (SD) and oil palms empty fruit bunch (OPEFB). The bacterial surface hydrophobic property of the bacterial CC was 60.3 ± 2.20 %, which suggested high bacterial cell attachment onto the carrier materials. The free bacterial CC exhibited the ability to produce exopolysaccharide (EPS) in minimal salt medium (MSM). The highest value of EPS produced by free bacteria CC was 61.3 ± 2.10 %. Due to their surface hydrophobic properties and ability to produce EPS, the bacterial CC was self-immobilized onto OPEFB and SD. The immobilized bacterial CC was then used to degrade crude oil hydrocarbons. The results showed that bacterial CC immobilized onto OPEFB and SD increased degradation of crude oil by 17.52 % and, 15.85 % respectively, at week 6 of incubation, and shortened the time to complete degradation by 25 % (from 8 to 6 weeks) compared to free bacterial CC. Immobilized bacterial CC enhanced biosurfactant production, as indicated by the emulsification index (E24%). Thus, the present study demonstrated that bacterial CC immobilized onto carrier materials increases crude oil degradation by increasing production of biosurfactants.

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Acknowledgments

The work described in this paper was supported by Exxon-Mobil STGL-008-2006 and the Malaysian Ministry of Science, Technology and Innovation (MOSTI), Escience Fund 06-01-02-SF0469.

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Authors and Affiliations

  1. Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia

    Mohammad Hazaimeh, Sahilah Abd Mutalib & Salmijah Surif

  2. Faculty of Health & Life Sciences, INTI International University, 71800, Nilai, Negeri Sembilan, Malaysia

    Palsan Sannasi Abdullah & Wong Kok Kee

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  1. Mohammad Hazaimeh
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  2. Sahilah Abd Mutalib
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  3. Palsan Sannasi Abdullah
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  4. Wong Kok Kee
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  5. Salmijah Surif
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Correspondence to Mohammad Hazaimeh.

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Hazaimeh, M., Abd Mutalib, S., Abdullah, P.S. et al. Enhanced crude oil hydrocarbon degradation by self-immobilized bacterial consortium culture on sawdust and oil palm empty fruit bunch. Ann Microbiol 64, 1769–1777 (2014). https://doi.org/10.1007/s13213-014-0821-3

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  • DOI: https://doi.org/10.1007/s13213-014-0821-3

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