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The production of poly(3-hydroxybutyrate) [P(3HB)] by a newly isolated Bacillus sp. ST1C using liquid waste from biodiesel production

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Abstract

A newly isolated poly(3-hydroxybutyrate) [P(3HB)] producing strain, ST1C, was identified as Bacillus aryabhattai based on its morphological, biochemical and molecular characteristics. It synthesized and accumulated relatively high amounts of P(3HB). The aim of this work was to establish if it could convert an inexpensive liquid waste product from the production of biodiesel, biodiesel liquid waste (BLW), to P(3HB). Using a mineral salt medium (MSM) containing 2.0 % (v/v) glycerol present in the BLW and both normal batch and a draw and fill culture method, B. aryabhattai ST1C produced a maximum P(3HB) content and biomass concentration of 72.31 % dry cell weight (DCW) and 7.24 g/L, respectively, over a 24 h cultivation period in the draw and fill cultivation method. From 24 h to the end of cultivation at 72 h both the P(3HB) content and the biomass concentrations continuously reduced. Concentrations of glycerol in the BLW in this MSM above 3.0 % (v/v) or from pure glycerol (PG) or with an added NaCl concentration of greater than 3.0 % significantly reduced both the maximum P(3HB) content and the biomass concentrations.

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Acknowledgments

We would like to acknowledge gratefully the Specialized R & D Center for Alternative Energy from Palm Oil and Oil Crops, Faculty of Engineering, Prince of Songkla University for the supply of their biodiesel liquid waste. This research was financially supported by Prince of Songkla University (SCI560117S) and the Development and Promotion of Science and Technology Talents Project (DPST) Scholarship, Thailand.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla, Thailand, 90112

    Wankuson Chanasit, Lukman Sueree & Kamontam Umsakul

  2. Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, Thailand, 90112

    Brian Hodgson

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  1. Wankuson Chanasit
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  2. Lukman Sueree
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  3. Brian Hodgson
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  4. Kamontam Umsakul
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Correspondence to Kamontam Umsakul.

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Chanasit, W., Sueree, L., Hodgson, B. et al. The production of poly(3-hydroxybutyrate) [P(3HB)] by a newly isolated Bacillus sp. ST1C using liquid waste from biodiesel production. Ann Microbiol 64, 1157–1166 (2014). https://doi.org/10.1007/s13213-013-0755-1

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