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Genetically modified Saccharomyces cerevisiae for one-step fermentation of bioalcohol using corncob as sole carbon source

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Abstract

The corncob is an important biomass for bioalcohol production. However, there is a minor but complicated pretreatment process before it is used for bioalcohol fermentation. In this study, three genetically modified Saccharomyces cerevisiae Y33 strains containing endoglucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (BG) genes were constructed. A one-step fermentation process was carried out with the recombinants using corncob as the sole carbon source. In a 3-L fermentation system, the concentration of alcohol reached 2.02 g/L and the concentration of glycerine reached 0.85 g/L after 96 h. The results prove that corncob powder can be utilized effectively by genetically modified Saccharomyces cerevisiae without any chemical pretreatment. The mixed recombinant Saccharomyces cerevisiae cells show effective synergism in the one-step fermentation system. It is feasible that corncob can be used as the sole carbon source in bioalcohol production with a one-step fermentation process.

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Acknowledgments

This work was supported by the following grants: National High Technology Research and Development Program ("863"Program: 2012AA020403, 2012AA02A701), Nation Natural Science Foundation of China (21076067), Program for New Century Excellent Talents in University (NCET-10-0153), Hubei Provincial Science Fund (2011CDA080, 2012DBA28001), Wuhan Science and Technology Bureau Program (2013060501010165).

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Authors and Affiliations

  1. Hubei Key Laboratory of Industrial Biotechnology, College of Life Science, Hubei University, Wuhan, 430062, People’s Republic of China

    Huiting Song, Jiashu Liu, Yang Liu, Huan Leng, Xu Li, Yong Zhao, Xiaosong Yang & Zhengbing Jiang

  2. College of Resources and Environmental Science, Hubei University, Wuhan, 430062, People’s Republic of China

    Huiting Song

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  1. Huiting Song
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  2. Jiashu Liu
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  3. Yang Liu
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  4. Huan Leng
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  5. Xu Li
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  6. Yong Zhao
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  7. Xiaosong Yang
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  8. Zhengbing Jiang
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Correspondence to Zhengbing Jiang.

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Huiting Song, Jiashu Liu and Yang Liu these authors contributed equally to this work

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Song, H., Liu, J., Liu, Y. et al. Genetically modified Saccharomyces cerevisiae for one-step fermentation of bioalcohol using corncob as sole carbon source. Ann Microbiol 64, 781–785 (2014). https://doi.org/10.1007/s13213-013-0714-x

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  • DOI: https://doi.org/10.1007/s13213-013-0714-x

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