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Overexpression of recombinant Nep1 in Escherichia coli and its use as a biological agent for control of Sinapis arvensis

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Abstract

Weeds are a significant part of the pests limiting crop production. Currently, chemical herbicides are widely used for weed control. Environment pollution and the rise of resistant strains highlight the need for new herbicides. Nep1 is a natural bio-herbicide protein which is an effective necrosis stimulant in dicotyledonous weeds. In this study, the cDNA encoding nep1 was isolated form Fusarium oxysporum, cloned and overexpressed in Escherichia coli. The Nep1 inclusion body was purified and refolded. For biological assay, the recombinant Nep1 was applied on Sinapis arvensis, as a chemical herbicide-resistant weed, and on Nicotiana tabacum, as a model plant. Our results show a significant necrosis on the leaves of S. arvensis and N. tabacum after spraying 50 μg/ml of the recombinant protein.

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Acknowledgments

The authors would like to thank Dr. Ali Mohammad Latifi for his valuable support.

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Authors and Affiliations

  1. Microbial Biotechnology Laboratory, Department of Microbiology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

    Hamid Moghimi & Javad Hamedi

  2. UTMC, University of Tehran Microorganisms Collection, Tehran, Iran

    Hamid Moghimi & Javad Hamedi

  3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Zargham Sepehrizadeh

  4. Biotechnology Department, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

    Hamideh Ofoghi

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  1. Hamid Moghimi
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  2. Javad Hamedi
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  3. Zargham Sepehrizadeh
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  4. Hamideh Ofoghi
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Correspondence to Javad Hamedi.

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Moghimi, H., Hamedi, J., Sepehrizadeh, Z. et al. Overexpression of recombinant Nep1 in Escherichia coli and its use as a biological agent for control of Sinapis arvensis . Ann Microbiol 63, 669–675 (2013). https://doi.org/10.1007/s13213-012-0517-5

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  • DOI: https://doi.org/10.1007/s13213-012-0517-5

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