Abstract
The purpose of this research was to isolate microorganisms from coffee fermentation processes and screen them for their potential to improve the flavor of Arabica coffee using a new approach that included pectin degradation ability and growth in mucilage broth. All of the studied microorganisms were isolated from 38 different samples of fresh coffee cherries, coffee mucilage and coffee pulp. A total of 262 microbial isolates were obtained and subjected to screening using pectinase screening agar medium for pectinolytic organisms. The results of the pectinase production test showed that 18 yeast isolates were found to produce pectinase that could degrade the pectin present in solid media. The sugar assimilation profiles and growth of selected strains in mucilage broth were studied. Therefore, 18 isolates from the selected yeasts were subjected to molecular identification by the use of 18S rRNA gene sequencing. The diversity of the yeast isolates was studied, and they were identified as Wickerhamomyces anomalus, Naganishia liquefaciens, Pichia kudriavzevii, Kazachstania naganishii and Kazachstania sp. Moreover, isolates SWU3YWP1-3, SWU3YSK9 and INFCY1-4 were used as a seed culture for Arabica coffee fermentation. The cupping sensory scores of the control (without yeast inoculation) and those inoculated with three isolated yeast strains that were determined by Q-Arabica Graders were 73.75, 84.75, 80.25 and 75.00, respectively. Unique flavors and aromas were detected. This is the first report of screening microorganisms from the Arabica coffee fermentation process by the combination of various properties with success in improving the quality of coffee beverage.
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Acknowledgements
This research was conducted in collaboration with UCC-K2 Co., Ltd., who provided assistance and contacted a coffee plantation to collect samples and conduct research by Mr. Meechai Amornpattanakul, President of the Thai Barista Association. This collaboration also included the Bank for Agriculture and Cooperatives, which supported this research. In addition, the researcher would like to thank the Royal Project Foundation for arranging contact with the coffee plantations in Chiang Mai under supervision for the researcher to travel to collect samples. In addition, this work was supported by the Kasetsart University Research and Development Institute (KURDI) grant no. FF(KU)18.64 under the coffee innovation research unit at Srinakharinwirot University grant no. 680/2563. Moreover, this work was funded by the Faculty of Science Srinakharinwirot University research fund Grant no. 491/2562.
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SK: methodology, data curation, formal analysis, investigation, writing—original draft, supervision. PS: methodology, experimenting. PT: review, manuscript revision, English editing. RM: methodology, experimenting. YM: methodology, experimenting. TP: experimenting. SS: review. KD: review. RJ: review. WL: writing—original draft, review and editing. NS: funding acquisition, project administration.
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Krajangsang, S., Seephin, P., Tantayotai, P. et al. New approach for screening of microorganisms from Arabica coffee processing for their ability to improve Arabica coffee flavor. 3 Biotech 12, 143 (2022). https://doi.org/10.1007/s13205-022-03203-5
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DOI: https://doi.org/10.1007/s13205-022-03203-5