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Characterization of maize miRNAs responsive to maize Iranian mosaic virus infection

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Abstract

MicroRNAs (miRNAs) play key regulatory roles in the plant’s response to biotic and abiotic stresses and have fundamental functions in plant–virus interactions. The study of changes in miRNAs in response to virus infection can provide molecular details for a better understanding of virus-host interactions. Maize Iranian mosaic virus (MIMV) infects maize and certain other poaceous plants but miRNA changes in response to MIMV infection are unknown. In the present study, we compared the miRNA profiles of MIMV-infected and uninfected maize and characterized their predicted roles in response to the virus. Small RNA sequencing of maize identified 257 conserved miRNAs of 26 conserved families in uninfected and MIMV-infected maize libraries. Among them, miR395, miR166 and miR156 family members were highly represented. Small RNA data were confirmed using RT-qPCR. In addition, 33 potential novel miRNAs were predicted. The data show that 13 miRNAs were up-regulated and 113 were down-regulated in response to MIMV infection. Several of those miRNAs are known to be important in the response to plant pathogens. To determine the potential roles of individual miRNAs in response to MIMV, miRNA targets, predicted interactions with circular RNAs and comparative transcriptome data were analyzed. The expression profiles of different miRNAs in response to MIMV provide novel insights into the roles of miRNAs in the interaction between MIMV and maize plants.

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Acknowledgements

AG was supported by a fellowship from the National Foundation of Elites, Shiraz University and Iranian Council of Centers of Excellence. This research was jointly supported by the University of Queensland and the Queensland Department of Agriculture and Fisheries. Fund for Small RNA-Seq and preliminary experiments was provided by Plant Virology Research Center, College of Agriculture, Shiraz University, Iran.

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Authors and Affiliations

  1. Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran

    Abozar Ghorbani, Keramatollah Izadpanah & Alireza Afsharifar

  2. Institute of Biotechnology, College of Agriculture, Shiraz University, Shiraz, Iran

    Ahmad Tahmasebi & Ali Moghadam

  3. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia

    Abozar Ghorbani & Ralf G. Dietzgen

Authors
  1. Abozar Ghorbani
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  2. Keramatollah Izadpanah
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  3. Ahmad Tahmasebi
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  4. Alireza Afsharifar
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  5. Ali Moghadam
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  6. Ralf G. Dietzgen
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Contributions

AG, KI, AA and RD designed the study; AG performed the experiments, AG, AT and AM analyzed the data and drafted the manuscript, all authors edited and approved the final version of the manuscripts.

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Correspondence to Abozar Ghorbani.

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The authors declare that they have no conflict of interest in the publication.

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Accession numbers: SAMN15248610 and SAMN15248611 (NCBI).

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Ghorbani, A., Izadpanah, K., Tahmasebi, A. et al. Characterization of maize miRNAs responsive to maize Iranian mosaic virus infection. 3 Biotech 12, 69 (2022). https://doi.org/10.1007/s13205-022-03134-1

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